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Titolo:
The quality of merC, a module of the mer mosaic
Autore:
Liebert, CA; Watson, AL; Summers, AO;
Indirizzi:
Univ Georgia, Dept Microbiol, Athens, GA 30602 USA Univ Georgia Athens GAUSA 30602 ia, Dept Microbiol, Athens, GA 30602 USA
Titolo Testata:
JOURNAL OF MOLECULAR EVOLUTION
fascicolo: 6, volume: 51, anno: 2000,
pagine: 607 - 622
SICI:
0022-2844(200012)51:6<607:TQOMAM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRAM-NEGATIVE BACTERIA; ORGANOMERCURIAL-RESISTANCE DETERMINANTS; ESCHERICHIA-COLI; THIOBACILLUS-FERROOXIDANS; NUCLEOTIDE-SEQUENCE; CODON USAGE; MOLECULAR CHARACTERIZATION; HOMOLOGOUS RECOMBINATION; DETOXIFICATION SYSTEM; CYSTEINE RESIDUES;
Keywords:
operon organization; mercury resistance; horizontal gene transfer; genome rearrangement; Chi-homologous recombination; codon usage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Summers, AO Univ Georgia, Dept Microbiol, 527 Biol Sci Bldg, Athens, GA 30602 USA Univ Georgia 527 Biol Sci Bldg Athens GA USA 30602 A 30602 USA
Citazione:
C.A. Liebert et al., "The quality of merC, a module of the mer mosaic", J MOL EVOL, 51(6), 2000, pp. 607-622

Abstract

We examined a region of high variability in the mosaic mercury resistance (mer) operon of natural bacterial isolates from the primate intestinal microbiota. The region between the merP and merA genes of nine mer loci was sequenced and either the merC, the merF, or no gene was present. Two novel merC genes were identified. Overall nucleotide diversity, pi (per 100 sites), of the merC gene was greater (49.63) than adjacent merP (35.82) and merA (32.58) genes. However, the consequences of this variability for the predicted structure of the MerC protein are limited and putative functional elements (metal-binding ligands and transmembrane domains) are strongly conserved,Comparison of codon usage of the merTP, merC, and merA genes suggests thatseveral merC genes are not coeval with their flanking sequences. Although evidence of homologous recombination within the very variable merC genes isnot apparent, the flanking regions have higher homologies than merC, and recombination appears to be driving their overall sequence identities higher. The synonymous codon usage bias (ENC) values suggest greater variability in expression of the merC gene than in flanking genes in six different bacterial hosts. We propose a model for the evolution of MerC as a host-dependent, adventitious module of the mer operon.

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Documento generato il 03/06/20 alle ore 09:47:48