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Titolo:
Impaired affinity maturation in Cr2(-/-) mice is rescued by adjuvants without improvement in germinal center development
Autore:
Wu, XB; Jiang, N; Fang, YF; Xu, CG; Mao, DL; Singh, J; Fu, YX; Molina, H;
Indirizzi:
Washington Univ, Sch Med, Dept Med, Div Rheumatol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 v Rheumatol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 l & Immunol, St Louis, MO 63110 USA Univ Chicago, Dept Pathol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 ago, Dept Pathol, Chicago, IL 60637 USA Vet Adm Med Ctr, St Louis, MO 63125 USA Vet Adm Med Ctr St Louis MO USA 63125 Adm Med Ctr, St Louis, MO 63125 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 6, volume: 165, anno: 2000,
pagine: 3119 - 3127
SICI:
0022-1767(20000915)165:6<3119:IAMICM>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICULAR DENDRITIC CELLS; PRIMARY IMMUNE-RESPONSE; COMPLEMENT RECEPTOR-1; B-CELLS; DEPENDENT ANTIGEN; ACQUIRED-IMMUNITY; DEFICIENT MICE; CR-1 CD35; GENERATION; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Molina, H Washington Univ, Sch Med, Dept Med, Div Rheumatol, Box 8045,660 S Euclid Ave, St Louis, MO 63110 USA Washington Univ Box 8045,660 S Euclid Ave St Louis MO USA 63110
Citazione:
X.B. Wu et al., "Impaired affinity maturation in Cr2(-/-) mice is rescued by adjuvants without improvement in germinal center development", J IMMUNOL, 165(6), 2000, pp. 3119-3127

Abstract

Cr2(-/-) mice have an impairment in humoral immunity, as shown by the decrease in the Ab titers against T cell-dependent Ags and abnormalities in germinal center formation. Germinal centers are present, but they are decreased in size and number, indicating problems in their development. In this study, we investigated whether this abnormality in germinal center developmentis associated with problems in the establishment of optimal affinity maturation and the generation of memory B cells, processes closely related to the germinal center reaction. We immunized the Cr2(-/-) animals with different Ags with or without adjuvants, We showed that, when immunized without adjuvants, complement receptors are absolutely required for optimal affinity maturation. Although limited affinity maturation is elicited in the Cr2(-/-)Ab response, it is decreased as compared with normal animals. Memory B cell generation is also impaired. In the presence of adjuvants, germinal center development in the Cr2(-/-) mice is still abnormal, as demonstrated by their decreased size and number. Surprisingly, adjuvants establish optimal affinity maturation and partially restore the amount of Ab produced during the primary response and memory B cell generation. However, adjuvants cannot improve the ability of follicular dendritic cells to retain Ags in the formof immune complexes. These observations indicate that immunization with inflammatory Ags offset some of the immunological abnormalities found in the Cr2(-/-) mice and show that optimal affinity maturation in the Cr2(-/-) mice can be achieved in the absence of normal germinal centers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:02:37