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Titolo:
Genetically modified bone marrow-derived vehicle cells site specifically deliver an anti-inflammatory cytokine to inflamed interstitium of obstructive nephropathy
Autore:
Yamagishi, H; Yokoo, T; Imasawa, T; Mitarai, T; Kawamura, T; Utsunomiya, Y;
Indirizzi:
Jikei Univ, Sch Med, Dept Internal Med, Div Nephrol & Hypertens,Minato Ku,Tokyo 1058461, Japan Jikei Univ Tokyo Japan 1058461 Hypertens,Minato Ku,Tokyo 1058461, Japan Jikei Univ, Sch Med, Dept Gene Therapy, Inst DNA Med, Tokyo 1058461, JapanJikei Univ Tokyo Japan 1058461 erapy, Inst DNA Med, Tokyo 1058461, Japan Saitama Med Sch, Dept Internal Med, Kawagoe, Saitama, Japan Saitama Med Sch Kawagoe Saitama Japan ernal Med, Kawagoe, Saitama, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 1, volume: 166, anno: 2001,
pagine: 609 - 616
SICI:
0022-1767(20010101)166:1<609:GMBMVC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN-1 RECEPTOR ANTAGONIST; SMOOTH MUSCLE ACTIN; EXPERIMENTAL HYDRONEPHROSIS; TUBULOINTERSTITIAL FIBROSIS; ADHESION MOLECULES; CRESCENTIC GLOMERULONEPHRITIS; LUPUS NEPHRITIS; RENAL-DISEASE; RAT-KIDNEY; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Utsunomiya, Y Jikei Univ, Sch Med, Dept Internal Med, Div Nephrol & Hypertens,Minato Ku,3-25-8 Nishi Shinbashi, Tokyo 1058461, Japan Jikei Univ 3-25-8 Nishi Shinbashi Tokyo Japan 1058461 Japan
Citazione:
H. Yamagishi et al., "Genetically modified bone marrow-derived vehicle cells site specifically deliver an anti-inflammatory cytokine to inflamed interstitium of obstructive nephropathy", J IMMUNOL, 166(1), 2001, pp. 609-616

Abstract

In this study, we used genetically modified bone marrow-derived CD11b(+)CD18(+) vehicle cells to deliver IL-1 receptor antagonist (n-1ra) for treatment of inflamed renal interstitium in an animal model of unilateral ureteralobstruction (UUO), Vehicle cells that expressed the ICAM-1 ligands, CD11b and CD18, were obtained from bone marrow cells of DBA/2j mice and adenovirally transduced with the IL-1ra gene or glucocerebrosidase (GC) gene ex vivo. In kidneys treated to develop UUO, levels of ICAM-1, IL-1 beta, and IL-1Rexpression increased within 3 days compared with contralateral untreated kidneys in the same mice, Similarly, the macrophage infiltration in the cortical interstitium increased after 3 days in UUO kidneys, but not untreated kidneys. After UUO developed, DBA/2j mice were injected i.v. with either IL-1ra(+) vehicle cells (IL-1ra-treated mice) or GC(+). vehicle cells (GC-treated mice) at 24 h after UUO, Six days after the injection of these vehiclecells, marked increase of CD11b(+) IL-1ra(+) vehicle cells was observed inthe ICAM-1-positive interstitium of UUO kidneys from IL-1ra-treated mice. In contrast, no CD11b(+) IL-1ra(+) cells appeared in ICAM-1-negative contralateral kidneys from these mice, Furthermore, the infiltration of macrophages (p < 0.001), expression of ICAM-1 (p < 0.005), and presence of alpha -smooth muscle actin (p = 0.005) in the interstitium of UUO kidneys were significantly decreased in IL-1ra-treated mice compared with GC-treated mice. These findings suggest that IL-1 may contribute to the development of renal interstitial injury and that our method can deliver a functioning gene encoding an antiinflammatory cytokine gene specifically at that site by interacting with local adhesion molecules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:20:51