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Titolo:
Localization of xenin-immunoreactive cells in the duodenal mucosa of humans and various mammals
Autore:
Anlauf, M; Weihe, E; Hartschuh, W; Hamscher, G; Feurle, GE;
Indirizzi:
Univ Marburg, Inst Anat & Zellbiol, Marburg, Germany Univ Marburg Marburg Germany rg, Inst Anat & Zellbiol, Marburg, Germany Univ Heidelberg, Hautklin, D-6900 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-6900 in, D-6900 Heidelberg, Germany Univ Bonn, Med Klin, D-5300 Bonn, Germany Univ Bonn Bonn Germany D-5300Univ Bonn, Med Klin, D-5300 Bonn, Germany DRK Krankenhaus, Neuwied, Germany DRK Krankenhaus Neuwied GermanyDRK Krankenhaus, Neuwied, Germany
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 12, volume: 48, anno: 2000,
pagine: 1617 - 1626
SICI:
0022-1554(200012)48:12<1617:LOXCIT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR MONOAMINE TRANSPORTER; ENDOCRINE-CELLS; XENOPUS-LAEVIS; PEPTIDE XENIN; GASTROINTESTINAL-TRACT; CHROMOGRANIN-A; IDENTIFICATION; INTESTINE; PRECURSOR; ISOFORMS;
Keywords:
xenin; endocrine cell; duodenal mucosa; gastric inhibitory polypeptide; chromogranin A; vesicular monoamine transporter; immunocytochemistry; immunoelectron microscopy; human; dog; Rhesus monkey;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Weihe, E Univ Marburg, Klinikum, Abt Mol Neurowissensch, Inst Anat & Zellbiol, D-35033 Marburg, Germany Univ Marburg Marburg Germany D-35033 , D-35033 Marburg, Germany
Citazione:
M. Anlauf et al., "Localization of xenin-immunoreactive cells in the duodenal mucosa of humans and various mammals", J HIST CYTO, 48(12), 2000, pp. 1617-1626

Abstract

Xenin is a 25-amino-acid peptide extractable from mammalian tissue. This peptide is biologically active. It stimulates exocrine pancreatic secretion and intestinal motility and inhibits gastric secretion of acid and food intake. Xenin circulates in the human plasma after meals. In this study, the cellular origin of xenin in the gastro-entero-pancreatic system of humans, Rhesus monkeys, and dogs was investigated by immunohistochemistry and immunoelectron microscopy. Sequence-specific antibodies against xenin detected specific endocrine cells in the duodenal and jejunal mucosa of all three species. These xenin-immunoreactive cells were distinct from enterochromaffin, somatostatin, motilin, cholecystokinin, neurotensin, and secretin cells, and comprised 8.8% of the chromogranin A-positive cells in the dog duodenum and 4.6% of the chromogranin A-positive cells in human duodenum. In all three species, co-localization of xenin was found with a subpopulation of gastric inhibitory polypeptide (GIP)-immunoreactive cells. Immunoelectron microscopy in the canine duodenal mucosa demonstrated accumulation of gold particles in round, homogeneous, and osmiophilic secretory granules with a closely adhering membrane of 187 +/- 19 nm diameter (mean +/- SEM). This cell type was found to be identical to the previously described canine CIP cell. Immunocytochemical expression of the peptide xenin in a subpopulation of chromogranin A-positive cells as wetI as the localization of xenin immunoreactivity in ultrastructurally characterized secretory granules permitted the identification of a novel endocrine cell type as the cellular source of circulating xenin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:09:08