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Titolo:
Hepatic stellate cells express Ca2+ pump-ATPase and Ca2+-Mg2+-ATPase in plasma membrane of caveolae
Autore:
Ogi, M; Yokomori, H; Inao, M; Oda, M; Ishii, H;
Indirizzi:
Kitasato Inst, Med Ctr Hosp, Pathol Lab, Kitamoto 3648501, Japan Kitasato Inst Kitamoto Japan 3648501 Pathol Lab, Kitamoto 3648501, Japan Kitasato Med Ctr Hosp, Dept Internal Med, Kitamoto, Japan Kitasato Med CtrHosp Kitamoto Japan Dept Internal Med, Kitamoto, Japan Saitama Med Sch, Dept Internal Med 3, Moroyama, Saitama 35004, Japan Saitama Med Sch Moroyama Saitama Japan 35004 royama, Saitama 35004, Japan Keio Univ, Sch Med, Dept Internal Med, Tokyo, Japan Keio Univ Tokyo Japan io Univ, Sch Med, Dept Internal Med, Tokyo, Japan
Titolo Testata:
JOURNAL OF GASTROENTEROLOGY
fascicolo: 12, volume: 35, anno: 2000,
pagine: 912 - 918
SICI:
0944-1174(200012)35:12<912:HSCECP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAT-STORING CELLS; RAT-LIVER; CALCIUM-PUMP; ECTO-ATPASE; PURIFICATION;
Keywords:
hepatic stellate cell; Ca2+-Mg2+-ATPase; Ca2+ pump-ATPase; immunofluorescence; ultrastructural localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Ogi, M Kitasato Inst, Med Ctr Hosp, Pathol Lab, 121-1 Arai, Kitamoto 3648501, Japan Kitasato Inst 121-1 Arai Kitamoto Japan 3648501 oto 3648501, Japan
Citazione:
M. Ogi et al., "Hepatic stellate cells express Ca2+ pump-ATPase and Ca2+-Mg2+-ATPase in plasma membrane of caveolae", J GASTRO, 35(12), 2000, pp. 912-918

Abstract

Intracytoplasmic free calcium ions (Ca2+) are maintained at a very low concentration in mammalian tissue by the extrusion of Ca2+ across a steep extracellular Ca2+ gradient, mainly through the activity of plasma membrane Ca2 pump-ATPase. The present study aimed to identify, by electron cytochemical and electron immunogold methods, the ultrastructural localizations of twotypes of plasma membrane Ca2+-ATPase: Ca2+-Mg2+-ATPase and Ca2+ pump-ATPase in hepatic stellate cells. Liver tissues and isolated hepatic stellate cells (HSCs) were studied. The ultrastructural localization of Ca2+-Mg2+-ATPase activity was examined by the electron cytochemical method of Ando. The localization of Ca2+ pump-ATPase was identified by immunofluorescence. The ultrastructural localization of Ca2+ Dump-ATPase was identified by the electron immunogold method. The cytochemical reaction products of Ca2+-Mg2+-ATPase activity were localized on the outer (cavity) side of the plasma membrane of caveolae. Immunofluorescence of Ca2+ pump-ATPase was seen as small dots along the eel edge in HSCs. Immunogold particles indicating the presence of Ca2+ pump-ATPase were identified on the inner (cytoplasmic) side of the plasma membrane of caveolae. We localized Ca2+ pump-ATPase on the inner side of the plasma membrane caveolae and Ca2+-Mg2+-ATPase on the outer leafletof the caveolar plasma membrane in stellate cells, suggesting that Ca2+ pump-ATPase may play a key role in the Ca2+ reflux.

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Documento generato il 25/11/20 alle ore 01:05:41