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Titolo:
The novel monoclonal antibody MH8-4 inhibiting cell motility recognizes integrin alpha 3: inverse of its expression with metastases in colon cancer
Autore:
Hashida, H; Takabayashi, A; Adachi, M; Imai, T; Kondo, K; Kohno, N; Yamaoka, Y; Miyake, M;
Indirizzi:
Kitano Hosp, Tazuke Kofukai Med Res Inst, Dept Oncol 5, Osaka 5308480, Japan Kitano Hosp Osaka Japan 5308480 Inst, Dept Oncol 5, Osaka 5308480, Japan Kitano Hosp, Tazuke Kofukai Med Res Inst, Dept Thorac Surg, Osaka 5308480,Japan Kitano Hosp Osaka Japan 5308480 t, Dept Thorac Surg, Osaka 5308480,Japan Kitano Hosp, Tazuke Kofukai Med Res Inst, Dept Surg, Osaka 5308480, Japan Kitano Hosp Osaka Japan 5308480 es Inst, Dept Surg, Osaka 5308480, Japan Kinki Univ, Sch Med, Dept Bacteriol, Osaka 5898511, Japan Kinki Univ Osaka Japan 5898511 Med, Dept Bacteriol, Osaka 5898511, Japan Ehime Univ, Sch Med, Dept Internal Med 2, Shigenobu, Ehime 7910295, Japan Ehime Univ Shigenobu Ehime Japan 7910295 Shigenobu, Ehime 7910295, Japan Kyoto Univ, Grad Sch Med, Dept Surg Gastroenterol, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 roenterol, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 1, volume: 18, anno: 2001,
pagine: 89 - 95
SICI:
1019-6439(200101)18:1<89:TNMAMI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRONECTIN RECEPTOR; CARCINOMA-CELLS; BREAST-CANCER; LUNG; GLYCOSYLATION; MEMBRANE; BINDING; ADENOCARCINOMA; INVASION; COLLAGEN;
Keywords:
monoclonal antibody; integrin; integrin alpha 3; colon cancer; metastasis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Miyake, M Kitano Hosp, Tazuke Kofukai Med Res Inst, Dept Oncol 5, 13-3 Kamiyamacho, Osaka 5308480, Japan Kitano Hosp 13-3 Kamiyamacho Osaka Japan 5308480 5308480, Japan
Citazione:
H. Hashida et al., "The novel monoclonal antibody MH8-4 inhibiting cell motility recognizes integrin alpha 3: inverse of its expression with metastases in colon cancer", INT J ONCOL, 18(1), 2001, pp. 89-95

Abstract

The molecular basis of cell motility is obviously highly complex and is considered to be controlled by a number of molecular systems including cell adhesion molecules, their receptors, cytoskeletal components, a junctional unit connecting cytoskeletal components and membrane receptors, and various peptide growth factors. The possible involvement of proteins at the cell surface in controlling cell motility has been systematically investigated. Previously, we have addressed this question using functional monoclonal antibodies (MAbs), which inhibit cell motility as probes. In order to further identify cell surface molecules involved in metastasis of gastrointestinal tumors, the present study utilized an approach based on the selection of a colon cancer cell line RPMI4788, which showed high motility out of a large number of human gastrointestinal tumor cell lines. MAb MH8-4 was established after immunization of mice with RPMI4788 and selected on the basis of inhibition of RPMI4788 cell migration in a transwell penetration assay. MH8-4 inhibited the phagokinetic tract motility of various cancer cell lines. A cDNA cloning revealed that MH8-4 recognized a specific protein structure, integrin alpha3. In order to determine whether these experimental results are of relevance with respect to actual human gastrointestinal tumors, we investigated integrin alpha3 expression in 40 colon cancers with distant metastases. Our immunohistochemical study showed that in almost 27.5% of the cases,the metastatic tumors had lower integrin alpha3 levels than their corresponding primary tumors. Moreover, there were no primary tumors with lower integrin alpha3 expression than their corresponding metastatic tumors. Our data suggest that low integrin alpha3 expression may be associated with the metastatic potential of certain colon cancers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:07:00