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Titolo:
Differential expression of three types of nitric oxide synthase in both infarcted and non-infarcted left ventricles after myocardial infarction in the rat
Autore:
Takimoto, Y; Aoyama, T; Keyamura, R; Shinoda, E; Hattori, R; Yui, Y; Sasayama, S;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 ovasc Med, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF CARDIOLOGY
fascicolo: 2-3, volume: 76, anno: 2000,
pagine: 135 - 145
SICI:
0167-5273(200011/12)76:2-3<135:DEOTTO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEART-FAILURE; TUMOR-NECROSIS-FACTOR; CARDIAC MYOCYTES; CONSCIOUS DOGS; BETA; ISCHEMIA;
Keywords:
nitric oxide synthase; myocardial infarction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Aoyama, T Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, 54 Kawaracho, Kyoto 6068507, Japan Kyoto Univ 54 Kawaracho Kyoto Japan 6068507 yoto 6068507, Japan
Citazione:
Y. Takimoto et al., "Differential expression of three types of nitric oxide synthase in both infarcted and non-infarcted left ventricles after myocardial infarction in the rat", INT J CARD, 76(2-3), 2000, pp. 135-145

Abstract

In the present report we investigated the differential expression of threetypes of nitric oxide synthase (NOS) in the left ventricle after myocardial infarction in rats. One, 3, 7, 14, 28 and 56 days (n=6-12 for each group)after ligation of a coronary artery, tissue samples were obtained from infarcted and non-infarcted tissues. The mRNA and protein levels of neuronal (n) NOS, endothelial (e) NOS and inducible (i) NOS were sequentially determined by semi-quantitative reverse transcription-polymerase chain reaction and Western blotting. Progressive left ventricular dilatation and gradual reduction in fractional shortening were confirmed by echocardiography. The expression levels of nNOS were significantly increased 1, 3 and 7 days post-infarct compared to those of sham-operated rats in both the infarcted (P<0.01) and non-infarcted regions (P<0.01). Immunohistochemical analysis showed that nNOS was localized in nerve fibers in the left ventricle and that the number of positive fibers after myocardial infarction had increased comparedto that in sham-operated rats. With regard to eNOS, no significant changesin expression levels were detected between infarcted hearts and sham-operated controls. The level of iNOS expression peaked three days post-infarct and then decreased in the infarcted tissue, whereas it increased one day post-infarct, peaked at 14 and 28 days post-infarct and was still elevated in the chronic stage in the ventricular septum. iNOS immunoreactivity was detected in spared cardiomyocytes and macrophages in the infarcted region, and in cardiomyocytes in the ventricular septum. The expressions of three typesof NOS were differentially regulated and iNOS produced in the non-infarcted region may contribute to the progression of heart failure after myocardial infarction in rats. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 21:59:46