Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Legionella pneumophila entry gene rtxA is involved in virulence
Autore:
Cirillo, SLG; Bermudez, LE; El-Etr, SH; Duhamel, GE; Cirillo, JD;
Indirizzi:
Univ Nebraska, Dept Vet & Biomed Sci, Lincoln, NE 68583 USA Univ NebraskaLincoln NE USA 68583 et & Biomed Sci, Lincoln, NE 68583 USA Calif Pacific Med Ctr, Kuzell Inst Arthrit & Infect Dis, San Francisco, CA94115 USA Calif Pacific Med Ctr San Francisco CA USA 94115 n Francisco, CA94115 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 1, volume: 69, anno: 2001,
pagine: 508 - 517
SICI:
0019-9567(200101)69:1<508:LPEGRI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEGIONNAIRES-DISEASE BACTERIUM; MYCOBACTERIUM-AVIUM COMPLEX; GRAM-NEGATIVE BACTERIA; PASTEURELLA-HAEMOLYTICA; ESCHERICHIA-COLI; HUMAN-MONOCYTES; GUINEA-PIGS; CYTO-TOXIN; INTRACELLULAR SURVIVAL; PHAGOSOME TRAFFICKING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Cirillo, JD Univ Nebraska, Dept Vet & Biomed Sci, 203 VBS,Fair & E Campus Loop, Lincoln, NE 68583 USA Univ Nebraska 203 VBS,Fair & E Campus Loop Lincoln NE USA 68583
Citazione:
S.L.G. Cirillo et al., "Legionella pneumophila entry gene rtxA is involved in virulence", INFEC IMMUN, 69(1), 2001, pp. 508-517

Abstract

Successful parasitism of host cells by intracellular pathogens involves adherence, entry, survival, intracellular replication, and cell-to-cell spread. Our laboratory has been examining the role of early events, adherence and entry, in the pathogenesis of the facultative intracellular pathogen Legionella pneumophila. Currently, the mechanisms used by L. pneumophila to gain access to the intracellular environment are not well understood. We have recently isolated three loci, designated enh1, enh2 and enh3, that are involved in the ability of L. pneumophila to enter host cells. One of the genespresent in the enh1 locus, rtxA, is homologous to repeats in structural toxin genes (RTX) found in many bacterial pathogens. RTX proteins from other bacterial species are commonly cytotoxic, and some of them have been shown to bind to beta (2) integrin receptors. In the current study, we demonstrate that the L. pneumophila rtxA gene is involved in adherence, cytotoxicity,and pore formation in addition to its role in entry. Furthermore, an rtxA mutant does not replicate as well as wild-type L. pneumophila in monocytes and is less virulent in mice. Thus, we conclude that the entry gene rtxA isan important virulence determinant in L. pneumophila and is likely to be critical for the production of Legionnaires' disease in humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:56:57