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Titolo:
vimA gene downstream of recA is involved in virulence modulation in Porphyromonas gingivalis W83
Autore:
Abaibou, H; Chen, Z; Olango, GJ; Liu, Y; Edwards, J; Fletcher, HM;
Indirizzi:
Loma Linda Univ, Sch Med, Dept Microbiol & Mol Genet, Loma Linda, CA 92350USA Loma Linda Univ Loma Linda CA USA 92350 ol Genet, Loma Linda, CA 92350USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 1, volume: 69, anno: 2001,
pagine: 325 - 335
SICI:
0019-9567(200101)69:1<325:VGDORI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEINASE LYS-GINGIPAIN; CYSTEINE PROTEINASE; ARG-GINGIPAIN; BACTEROIDES-GINGIVALIS; NUCLEOTIDE-SEQUENCE; EXPRESSION; CLONING; DNA; HEMAGGLUTINATION; PROTEASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Fletcher, HM Loma Linda Univ, Sch Med, Dept Microbiol & Mol Genet, Loma Linda, CA 92350USA Loma Linda Univ Loma Linda CA USA 92350 a Linda, CA 92350USA
Citazione:
H. Abaibou et al., "vimA gene downstream of recA is involved in virulence modulation in Porphyromonas gingivalis W83", INFEC IMMUN, 69(1), 2001, pp. 325-335

Abstract

A 0.9-kb open reading frame encoding a unique 32-kDa protein was identified downstream of the recA gene of Porphyromonas gingivalis. Reverse transcription PCR and Northern blot analysis showed that both the recA gene and this open reading frame are part of the same transcriptional unit. This clonedfragment was insertionally inactivated using the ermF-ermAM antibiotic resistance cassette to create a defective mutant by allelic exchange. When plated on Brucella blood agar, the mutant strain, designated P. gingivalis FLL92, was non-black pigmented and showed significant reduction in beta-hemolysis compared with the parent strain, P. gingivalis W83. Arginine- and lysine-specific cysteine protease activities, which were mostly soluble, were approximately 90% lower than that of the parent strain. Expression of the rgpA, rgpB, and kgp protease genes was the same in P. gingivalis FLL92 as in the wild-type strain. In contrast to the parent strain, P. gingivalis FLL92 showed increased autoaggregration in addition to a significant reduction inhemagglutinating and hemolysin activities. In in vivo experiments using a mouse model, P. gingivalis FLL92 was dramatically less virulent than the parent strain. A molecular survey of this mutant and the parent strain using all known P. gingivalis insertion sequence elements as probes suggested that no intragenomic changes due to the movement of these elements have occurred in P. gingivalis FLL92. Taken together, these results suggest that the recA downstream gene, designated vimA (virulence-modulating gene), plays an important role in virulence modulation in P. gingivalis W83, possibly representing a novel posttranscriptional or translational regulation of virulence factors in P. gingivalis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 15:54:07