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Titolo:
T-cell antigen receptors in Atlantic cod (Gadus morhua L.): structure, organisation and expression of TCR alpha and beta genes
Autore:
Wermenstam, NE; Pilstrom, L;
Indirizzi:
Univ Uppsala, BMC, Dept Cell & Mol Biol, Immunol Programme, S-75124 Uppsala, Sweden Univ Uppsala Uppsala Sweden S-75124 l Programme, S-75124 Uppsala, Sweden
Titolo Testata:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
fascicolo: 2, volume: 25, anno: 2001,
pagine: 117 - 135
SICI:
0145-305X(200103)25:2<117:TARIAC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DICENTRARCHUS-LABRAX L; CHANNEL CATFISH; TELEOST FISH; IMMUNOCYTOCHEMICAL DETECTION; LYMPHOCYTE HETEROGENEITY; NUCLEOTIDE-SEQUENCES; TRANSMEMBRANE REGION; EXTENSIVE DIVERSITY; MEXICAN AXOLOTL; LIGHT-CHAIN;
Keywords:
T-cell antigen receptor; TCR alpha; TCR beta; Ig-superfamily; Atlantic cod; Gadus morhua;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Pilstrom, L Univ Uppsala, BMC, Dept Cell & Mol Biol, Immunol Programme, Box 596, S-75124 Uppsala, Sweden Univ Uppsala Box 596 Uppsala Sweden S-751244 Uppsala, Sweden
Citazione:
N.E. Wermenstam e L. Pilstrom, "T-cell antigen receptors in Atlantic cod (Gadus morhua L.): structure, organisation and expression of TCR alpha and beta genes", DEV COMP IM, 25(2), 2001, pp. 117-135

Abstract

By using short degenerate primers complementing conserved T-cell antigen receptor (TCR) variable and constant region segments for PCR, we were able to isolate putative TCR alpha and beta chain full length cDNAs in Atlantic cod. The Ver and VP domains have the canonical features of known teleost andmammalian TCR V domains, including conserved residues in the beginning of FR2 and at the end of FR3. The J alpha and J beta region possess the conserved Phe-GIy-X-Gly motif found in nearly all TCR and immunoglobulin light chain J regions. Similar to other vertebrates, the Atlantic cod Ca: and C beta sequences exhibit distinct immunoglobulin, connecting peptide, transmembrane and cytoplasmic regions. The Atlantic cod C beta sequence lacks a cysteine in its connecting peptide region, but other motifs proposed to be important for dimerisation and cell surface expression are observed. Four different cod C beta sequences were identified, two of which share 3' untranslated regions different from one of the other two sequences, suggesting the existence of isotypic gene variants of C beta. Based on Southern blot analyses, the TCR alpha and beta gene loci appear to be arranged in translocon organisation las opposed to multicluster) with multiple V gene segments, some (D) and J gene segments and a single or few C gene segments. Northern blot analyses show expression of the TCR alpha and beta chains in thymus, spleen and head kidney, expression of the TCR beta chain was also detected in the ovary. Interestingly, no expression was detected in intestine even though the existence of T-cells in intestine has been proposed in other teleost species. (C) 2000 Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 21:56:48