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Titolo:
Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) andsomatic cell hybrid analysis
Autore:
Lindgren, G; Breen, M; Godard, S; Bowling, A; Murray, J; Scavone, M; Skow, L; Sandberg, K; Guerin, G; Binns, M; Ellegren, H;
Indirizzi:
Uppsala Univ, Dept Evolutionary Biol, S-75236 Uppsala, Sweden Uppsala Univ Uppsala Sweden S-75236 ionary Biol, S-75236 Uppsala, Sweden Anim Hlth Trust, Newmarket CB8 7UU, Suffolk, England Anim Hlth Trust Newmarket Suffolk England CB8 7UU 8 7UU, Suffolk, England INRA, Ctr Rech Jouy En Josas, Lab Genet Biochim & Cytogenet, F-78352 Jouy En Josas, France INRA Jouy En Josas France F-78352 togenet, F-78352 Jouy En Josas, France Univ Calif Davis, Sch Vet Med, Vet Genet Lab, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 d, Vet Genet Lab, Davis, CA 95616 USA Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 s, Dept Anim Sci, Davis, CA 95616 USA Univ Calif Davis, Sch Vet Med, Dept Reprod & Populat Hlth, Davis, CA 95616USA Univ Calif Davis Davis CA USA 95616 od & Populat Hlth, Davis, CA 95616USA Texas A&M Univ, Dept Vet Anat & Publ Hlth, College Stn, TX 77843 USA TexasA&M Univ College Stn TX USA 77843 l Hlth, College Stn, TX 77843 USA Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75124 Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden S-75124 net, S-75124 Uppsala, Sweden
Titolo Testata:
CHROMOSOME RESEARCH
fascicolo: 1, volume: 9, anno: 2001,
pagine: 53 - 59
SICI:
0967-3849(2001)9:1<53:MO1HGB>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
2ND-GENERATION LINKAGE MAP; MARKERS; GENOME; SYNTENY; MICROSATELLITES; IDENTIFICATION; CHROMOSOMES;
Keywords:
horse; physical mapping; type I markers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Lindgren, G Uppsala Univ, Dept Evolutionary Biol, Norbyvagen 18D, S-75236 Uppsala, Sweden Uppsala Univ Norbyvagen 18D Uppsala Sweden S-75236 la, Sweden
Citazione:
G. Lindgren et al., "Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) andsomatic cell hybrid analysis", CHROMOS RES, 9(1), 2001, pp. 53-59

Abstract

We report fluorescence in-situ hybridization (FISH) and somatic cell hybrid mapping data for 13 different horse genes (ANP, CD2, CLU, CRISP3, CYP17, FGG, IL1RN, IL10, MMP13, PRM1, PTGS2, TNFA and TP53). Primers for PCR amplification of intronic or untranslated regions were designed from horse-specific DNA or mRNA sequences in GenBank. Two different horse bacterial artificial chromosome (BAC) libraries were screened with PCR for clones containingthese 13 Type I loci, nine of which were found in the libraries. BAC clones were used as probes in dual colour FISH to confirm their precise chromosomal origin. The remaining four genes were mapped in a somatic cell hybrid panel. All chromosomal assignments except one were in agreement with human-horse ZOO-FISH data and revealed new and more detailed information on the equine comparative map. CLU was mapped by synteny to ECA2 while human-horse ZOO-FISH data predicted that CLU would be located on ECA9. The assignment ofIL1RN permitted analysis of gene order conservation between HSA2 and ECA15, which identified that an event of inversion had occurred during the evolution of these two homologous chromosomes.

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Documento generato il 13/07/20 alle ore 17:46:03