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Titolo:
Quantification of cytomegalovirus DNA in BAL fluid - A longitudinal study in lung transplant recipients
Autore:
Riise, GC; Andersson, R; Bergstrom, T; Lundmark, A; Nilsson, FN; Olofsson, S;
Indirizzi:
Sahlgrens Univ Hosp, Dept Resp Med, SE-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden SE-41345 -41345 Gothenburg, Sweden Sahlgrens Univ Hosp, Dept Infect Dis, SE-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden SE-41345 -41345 Gothenburg, Sweden Sahlgrens Univ Hosp, Dept Virol, SE-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden SE-41345 -41345 Gothenburg, Sweden Sahlgrens Univ Hosp, Dept Cardiothorac Surg, SE-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden SE-41345 -41345 Gothenburg, Sweden
Titolo Testata:
CHEST
fascicolo: 6, volume: 118, anno: 2000,
pagine: 1653 - 1660
SICI:
0012-3692(200012)118:6<1653:QOCDIB>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; BRONCHOALVEOLAR LAVAGE; WORKING FORMULATION; DISEASE; BLOOD; METHYLPREDNISOLONE; STANDARDIZATION; NOMENCLATURE; DYSFUNCTION; GANCICLOVIR;
Keywords:
BAL; cytomegalovirus; lung transplantation; quantitative polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Riise, GC Sahlgrens Univ Hosp, Dept Resp Med, SE-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden SE-41345 henburg, Sweden
Citazione:
G.C. Riise et al., "Quantification of cytomegalovirus DNA in BAL fluid - A longitudinal study in lung transplant recipients", CHEST, 118(6), 2000, pp. 1653-1660

Abstract

Study objectives: Cytomegalovirus (CMV) infection is common in patients receiving solid organ transplants, and it is associated with increased morbidity as well as risk for development of chronic rejection. A rapid and sensitive diagnostic method would improve the therapeutic management of CMV infection, including the monitoring of treatment effects. We investigated whether longitudinal determinations of CMV DNA quantities in BAL fluid could be useful for this purpose. Design: CMV DNA levels in 340 BAL samples from 35 consecutive lung transplant recipients were studied during a median of 18 months. Seventeen (49%) of the patients developed CMV disease with pneumonitis. Twenty-seven CMV disease episodes were diagnosed. Results: Patients with CMV disease had a significantly higher mean level of CMV copies per milliliter BAL fluid (1,120 +/- 4,379) compared with thosewithout (180 +/- 1,177, p < 0.01). Viral load as well as acute rejection requiring treatment (<greater than or equal to> A2) were independent risk factors associated with CMV disease. Differences between the groups concerning HLA-DR matching, basic immunosuppressive therapy, and CMV serologic status D/R -/+ vs D/R +/+ were not significant. A diagnostic definition of normality based on the mean level of all episodes without CMV disease +2 SD would discriminate only 9 of the 27 CMV episodes. Conclusions: Although the viral load is increased during episodes of clinical CMV disease in lung transplant recipients, the quantitative PCR assessment of CMV DNA in BAL fluid is not discriminative enough to be useful as a diagnostic tool for CMV disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 05:26:46