Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Localisation of 26S proteasomes with different subunit composition in insect muscles undergoing programmed cell death
Autore:
Low, P; Hastings, RA; Dawson, SP; Sass, M; Billett, MA; Mayer, RJ; Reynolds, SE;
Indirizzi:
Lorand Eotvos Univ, Dept Gen Zool, H-1445 Budapest, Hungary Lorand Eotvos Univ Budapest Hungary H-1445 ool, H-1445 Budapest, Hungary Univ Nottingham, Sch Biomed Sci, Nottingham NG7 2RD, England Univ Nottingham Nottingham England NG7 2RD , Nottingham NG7 2RD, England Univ Bath, Sch Biol & Biochem, Bath BA2 7AY, Avon, England Univ Bath BathAvon England BA2 7AY Biochem, Bath BA2 7AY, Avon, England
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 12, volume: 7, anno: 2000,
pagine: 1210 - 1217
SICI:
1350-9047(200012)7:12<1210:LO2PWD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAT-BINDING-PROTEIN; MANDUCA-SEXTA; REGULATORY PARTICLE; TRANSCRIPTIONAL ACTIVATOR; INTERSEGMENTAL MUSCLES; DEVELOPMENTAL-CHANGES; TOBACCO HORNWORM; SKELETAL-MUSCLE; YEAST; COMPLEX;
Keywords:
programmed cell death; tobacco hornworm; Manduca sexta; proteasome regulatory ATPase subunit; muscle degradation; immunogold method;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Low, P Lorand Eotvos Univ, Dept Gen Zool, POB 330, H-1445 Budapest, Hungary Lorand Eotvos Univ POB 330 Budapest Hungary H-1445 dapest, Hungary
Citazione:
P. Low et al., "Localisation of 26S proteasomes with different subunit composition in insect muscles undergoing programmed cell death", CELL DEAT D, 7(12), 2000, pp. 1210-1217

Abstract

The 26S proteasome is a large multisubunit complex involved in degrading both cytoplasmic and nuclear proteins. We have investigated the subcellular distribution of four regulatory ATPase subunits (S6 (TBP7/MS73), S6' (TBP1), S7 (MSS1), and S10b (SUG2)) together with components of 20S proteasomes in the intersegmental muscles (ISM) of Manduca sexta during developmentally programmed cell death (PCD). Immunogold electron microscopy shows that S6 is located in the heterochromatic part of nuclei of ISM fibres. S6' is present in degraded material only outside intact fibres. S7 can be detected in nuclei, cytoplasm and also in degraded material, S10b, on the other hand, isinitially found in nuclei and subsequently in degraded cytoplasmic locations during PCD. 20S proteasomes are present in all areas where ATPase subunits are detected, consistent with the presence of intact 26S proteasomes. These results are discussed in terms of heterogeneity of 26S proteasomes, 26Sproteasome disassembly and the possible role of ATPases in non-proteasome complexes in the process of PCD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 05:31:35