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Titolo:
Hydrodynamic characterisation of the exopolysaccharide from the halophiliccyanobacterium Aphanothece halophytica GR02: a comparison with xanthan
Autore:
Morris, GA; Li, P; Puaud, M; Liu, Z; Mitchell, JR; Harding, SE;
Indirizzi:
Univ Nottingham, Sch Biosci, Sutton Bonington LE12 5RD, England Univ Nottingham Sutton Bonington England LE12 5RD gton LE12 5RD, England Nanjing Univ, Dept Biol, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China Nanjing Univ Nanjing Peoples R China 210093 jing 210093, Peoples R China
Titolo Testata:
CARBOHYDRATE POLYMERS
fascicolo: 3, volume: 44, anno: 2001,
pagine: 261 - 268
SICI:
0144-8617(200103)44:3<261:HCOTEF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXOCELLULAR POLYSACCHARIDE; CYANOSPIRA-CAPSULATA; ANALYTICAL ULTRACENTRIFUGE; SEDIMENTATION EQUILIBRIUM; MOLECULAR-WEIGHT; CONFORMATION;
Keywords:
bacterial exopolysaccharide; Aphanothece halophytica; xanthan-like; hydrodynamic characterisation; guanidine hydrochloride; cross-equation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Morris, GA Univ Nottingham, Sch Biosci, Sutton Bonington LE12 5RD, EnglandUniv Nottingham Sutton Bonington England LE12 5RD RD, England
Citazione:
G.A. Morris et al., "Hydrodynamic characterisation of the exopolysaccharide from the halophiliccyanobacterium Aphanothece halophytica GR02: a comparison with xanthan", CARBOHY POL, 44(3), 2001, pp. 261-268

Abstract

A bacterial exopolysaccharide from Aphanothece halophytica GR02 (AH-EPS) solubilised in buffered 1 M guanidine hydrochloride, GuHCl was studied usingcapillary viscometry, double gap viscometry, sedimentation velocity and sedimentation equilibrium using analytical ultracentrifugation. Results from capillary viscometry indicate that the exopolysaccharide is xanthan-like inits shear thinning properties. This was confirmed by double gap viscometry. Sedimentation velocity (distribution of apparent sedimentation coefficients) confirmed that the AH-EPS solution was reasonably homogeneous and a hyper-sharp boundary indicated that AH-EPS is a rigid/extra-rigid rod type polysaccharide. Sedimentation equilibrium confirmed the high molecular weight indicated by viscosity measurements. in order to ascertain whether the solution physical properties of AH-EPS are xanthan-like under the conditions studied, all the above experiments were repeated on a similarly treated xanthan solution in the same solvent. Results indicate that AH-EPS is indeed xanthan-like in its physical properties at least in the system studied. Xanthan properties in GuHCl do not appear too different from those studied in more traditional solvents. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 04/12/20 alle ore 11:57:51