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Titolo:
Fabrication of a micro-channel chip for electrophoresis by using a Photosensitive epoxy resin as an etching mask
Autore:
Shiraishi, H; Kawanaka, S; Matsuda, T; Tabata, O; Ikeda, S;
Indirizzi:
Ritsumeikan Univ, Fac Sci & Engn, Kusatsu, Shiga 5258577, Japan Ritsumeikan Univ Kusatsu Shiga Japan 5258577 usatsu, Shiga 5258577, Japan Ritsumeikan Univ, SR Ctr, Kusatsu, Shiga 5258577, Japan Ritsumeikan Univ Kusatsu Shiga Japan 5258577 usatsu, Shiga 5258577, Japan
Titolo Testata:
BUNSEKI KAGAKU
fascicolo: 12, volume: 49, anno: 2000,
pagine: 1037 - 1041
SICI:
0525-1931(200012)49:12<1037:FOAMCF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
JPN
Soggetto:
CAPILLARY ELECTROPHORESIS;
Keywords:
micro-channel glass chip; electrophoresis; photosensitive epoxy resin; laser-induced fluorescence detection; fluorescein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
6
Recensione:
Indirizzi per estratti:
Indirizzo: Shiraishi, H Ritsumeikan Univ, Fac Sci & Engn, 1-1-1 Noji Higashi, Kusatsu, Shiga 5258577, Japan Ritsumeikan Univ 1-1-1 Noji Higashi Kusatsu Shiga Japan 5258577
Citazione:
H. Shiraishi et al., "Fabrication of a micro-channel chip for electrophoresis by using a Photosensitive epoxy resin as an etching mask", BUNSEKI KAG, 49(12), 2000, pp. 1037-1041

Abstract

In this paper, we present a new fabrication method that uses a UV-sensitive epoxy resin as an etching mask for a micro-channel electrophoresis chip. We have used it to separate fluorescein and fluorescein-5-sulfonic acid. The device was fabricated in a 50 mm square of soda glass by using micro-lithographic patterning and wet etching techniques. The proposed method does not need for plating and etching of Au/Cr masks. An etched bottom plate was covered with a top plate drilled access holes into it and permanently bondedin an oven by heating at 560 similar to 580 degreesC. The chip had a separation channel and a sample injection channel. The separation channel was 80mum wide and 20 mum deep. Using a micro-fabricated chip with a separation length of 10 mm and a separation voltage of 800 V, the electrophoresis of afluorescein and fluorescein-5-sulfonic acid mixture gave two well-separated peaks. Detection was achieved using a laser-induced fluorescence microscope. The HETP of this chip was 3 similar to 4 mum for fluorescence samples, and a separation coefficient of 2.2 was realized. The proposed fabrication method is very simple and easy, and has given separation and detection results for fluorescein derivatives.

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Documento generato il 26/01/20 alle ore 10:25:35