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Titolo:
Surfactant protein A and lipid are internalized via the coated-pit pathwayby type II pneumocytes
Autore:
Stevens, PA; Wissel, H; Zastrow, S; Sieger, D; Zimmer, KP;
Indirizzi:
Humboldt Univ, Univ Childrens Hosp, Clin Neonatol, D-10098 Berlin, GermanyHumboldt Univ Berlin Germany D-10098 n Neonatol, D-10098 Berlin, Germany Univ Munster, Univ Childrens Hosp, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 rens Hosp, D-48149 Munster, Germany
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 1, volume: 280, anno: 2001,
pagine: L141 - L151
SICI:
1040-0605(200101)280:1<L141:SPAALA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED ENDOCYTOSIS; APOPROTEIN SP-A; PULMONARY SURFACTANT; LAMELLAR BODIES; INTRACELLULAR POTASSIUM; HUMAN FIBROBLASTS; HIGH-AFFINITY; EGF RECEPTOR; RAT LUNG; CELLS;
Keywords:
early endosome; lamellar body; coated vesicle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Stevens, PA Humboldt Univ, Univ Childrens Hosp, Clin Neonatol, Charite Campus Mitte,Schumannstr 20-21, D-10098 Berlin, Germany Humboldt Univ Charite Campus Mitte,Schumannstr 20-21 Berlin Germany D-10098
Citazione:
P.A. Stevens et al., "Surfactant protein A and lipid are internalized via the coated-pit pathwayby type II pneumocytes", AM J P-LUNG, 280(1), 2001, pp. L141-L151

Abstract

Surfactant protein (SP) A and SP-A-mediated lipid uptake by isolated type II cells were investigated with biochemical and morphological methods. Inhibition of coated-pit function by potassium depletion severely reduced both SP-A and SP-A-mediated lipid internalization. Lipid uptake in the absence of SP-A was not affected. With confocal laser scanning microscopy and immunoelectron microscopy, SP-A and lipid predominantly (60%) colocalized in intracellular vesicles carrying early endosomal markers (EEA1) 5 min after endocytosis but were negative for the late endosomal or lysosomal marker LAMP-1. As estimated by subcellular fractionation, at this time point, 23% of theinternalized SP-A and 45% of internalized lipid were localized within light (<0.38 M sucrose) fractions, which contain lamellar bodies and are positive for EEA1. The remaining label was predominantly found within EEA1-positive and plasma membrane-containing subfractions (<greater than or equal to>1M sucrose). We suggest that in isolated type II cells in vitro, SP-A and lipid are taken up together via the coated-pit pathway and that at early time points, both components reside in the same early endosomal compartment.

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Documento generato il 21/10/20 alle ore 11:03:57