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Titolo:
Prostaglandin E-2-induced interleukin-6 release by a human airway epithelial cell line
Autore:
Tavakoli, S; Cowan, MJ; Benfield, T; Logun, C; Shelhamer, JH;
Indirizzi:
NIH, Ctr Clin, Dept Crit Care Med, Bethesda, MD 20892 USA NIH Bethesda MDUSA 20892 lin, Dept Crit Care Med, Bethesda, MD 20892 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 1, volume: 280, anno: 2001,
pagine: L127 - L133
SICI:
1040-0605(200101)280:1<L127:PEIRBA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLATELET-ACTIVATING-FACTOR; PROTEIN-KINASE-C; EP2 SUBTYPE; ALVEOLAR MACROPHAGES; ARACHIDONIC-ACID; T-CELLS; RECEPTOR; EXPRESSION; GENERATION; CLONING;
Keywords:
cytokines; eicosanoids; lung inflammation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Shelhamer, JH NIH, Ctr Clin, Dept Crit Care Med, Bldg 10,Rm 7-D-43, Bethesda, MD 20892 USA NIH Bldg 10,Rm 7-D-43 Bethesda MD USA 20892 da, MD 20892 USA
Citazione:
S. Tavakoli et al., "Prostaglandin E-2-induced interleukin-6 release by a human airway epithelial cell line", AM J P-LUNG, 280(1), 2001, pp. L127-L133

Abstract

Human airway epithelial cell release of interleukin (IL)-6 in response to lipid mediators was studied in an airway cell line (BEAS-2B). Prostaglandin(PG) E-2 (10(-7) M) treatment caused an increase in IL-6 release at 2, 4, 8, and 24 h. IL-6 release into the culture medium at 24 h was 3,396 +/- 306vs. 1,051 +/- 154 pg/ml (PGE(2)-treated cells vs. control cells). PGE(2) (10(-7) to 10(-10) M) induced a dose-related increase in IL-6 release at 24 h. PGF(2 alpha) (10(-6) M) treatment caused a similar effect to that of PGE(2) (10(-7) M). PGE(2) analogs with relative selectivity for PGE2 receptor subtypes were studied. Sulprostone, a selective agonist for the EP-3 receptor subtype had no effect on IL-6 release. 11-Deoxy-16,16-dimethyl-PGE(2,) an EP-2/4 agonist, and 17-phenyl trinor PGE(2), an agonist selective for theEP-1 > EP-3 receptor subtype (10(-6) to 10(-8) M), caused dose-dependent increases in IL-6 release. 8-Bromo-cAMP treatment resulted in dose-related increases in IL-6 release. RT-PCR of BEAS-2B cell mRNA demonstrated mRNA forEP-1, EP-2, and EP-4 receptors. After PGE(2) treatment, increases in IL-6 mRNA were noted at 4 and 18 h. Therefore, PGE(2) increases airway epithelial cell IL-6 production and release.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:54:49