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Titolo:
Extracellular cyclic ADP-ribose potentiates ACh-induced contraction in bovine tracheal smooth muscle
Autore:
Franco, L; Bruzzone, S; Song, PF; Guida, L; Zocchi, E; Walseth, TF; Crimi, E; Usai, C; De Flora, A; Brusasco, V;
Indirizzi:
Univ Naples Federico II, CNR, Biocrystallog Ctr, I-80134 Naples, Italy Univ Naples Federico II Naples Italy I-80134 Ctr, I-80134 Naples, Italy Univ Genoa, Dept Expt Med, Biochem Sect, I-16132 Genoa, Italy Univ Genoa Genoa Italy I-16132 t Med, Biochem Sect, I-16132 Genoa, Italy Univ Genoa, Dept Motor Sci, I-16132 Genoa, Italy Univ Genoa Genoa Italy I-16132 noa, Dept Motor Sci, I-16132 Genoa, Italy Natl Res Council, Inst Cybernet & Biophys, I-16149 Genoa, Italy Natl Res Council Genoa Italy I-16149 net & Biophys, I-16149 Genoa, Italy Univ Minnesota, Dept Pharmacol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rmacol, Minneapolis, MN 55455 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 1, volume: 280, anno: 2001,
pagine: L98 - L106
SICI:
1040-0605(200101)280:1<L98:ECAPAC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ERYTHROCYTES; CALCIUM RELEASE; OUTER SURFACE; HUMAN CD38; CYCLASE; GLYCOHYDROLASE; HYDROLYSIS; PROTEIN; CELLS; CA2+;
Keywords:
acetylcholine; CD38; nicotinamide adenine dinucleotide/cyclic adenosine 5 '-diphosphate-ribose-mediated paracrine mechanisms; calcium-related contraction of tracheal strips; adenosine 5 '-diphosphate-ribosyl cyclase; cyclic adenosine 5 '-diphosphate-ribose hydrolase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: De Flora, A Univ Genoa, DIMES, Biochem Sect, Viale Benedetto XV 1, Genoa, Italy Univ Genoa Viale Benedetto XV 1 Genoa Italy 1, Genoa, Italy
Citazione:
L. Franco et al., "Extracellular cyclic ADP-ribose potentiates ACh-induced contraction in bovine tracheal smooth muscle", AM J P-LUNG, 280(1), 2001, pp. L98-L106

Abstract

Cyclic ADP-ribose (cADPR), a universal calcium releaser, is generated fromNAD(+) by an ADP-ribosyl cyclase and is degraded to ADP-ribose by a cADPR hydrolase. In mammals, both activities are expressed as ectoenzymes by the transmembrane glycoprotein CD38. CD38 was identified in both epithelial cells and smooth myocytes isolated from bovine trachea. Intact tracheal smoothmyocytes (TSMs) responded to extracellular cADPR (100 muM) with an increase in intracellular calcium concentration ([Ca2+](i)) both at baseline and after acetylcholine (ACh) stimulation. The nonhydrolyzable analog 3-deaza-cADPR (10 nM) elicited the same effects as cADPR, whereas the cADPR antagonist 8-NH2-cADPR (10 muM) inhibited both basal and ACh-stimulated [Ca2+](i) levels. Extracellular cADPR or 3-deaza-cADPR caused a significant increase ofACh-induced contraction in tracheal smooth muscle strips, whereas 8-NH2-cADPR decreased it. Tracheal mucosa strips, by releasing NAD(+), enhanced [Ca2+](i) in isolated TSMs, and this increase was abrogated by either NAD(+)-ase or 8-NH2-cADPR. These data suggest the existence of a paracrine mechanism whereby mucosa-released extracellular NAD(+) plays a hormonelike functionand cADPR behaves as second messenger regulating calcium-related contractility in TSMs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 13:10:53