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Titolo:
Monocytes recruited into the alveolar air space of mice show a monocytic phenotype but upregulate CD14
Autore:
Maus, U; Herold, S; Muth, H; Maus, R; Ermert, L; Ermert, M; Weissmann, N; Rosseau, S; Seeger, W; Grimminger, F; Lohmeyer, J;
Indirizzi:
Univ Giessen, Dept Internal Med, D-35392 Giessen, Germany Univ Giessen Giessen Germany D-35392 ernal Med, D-35392 Giessen, Germany Univ Giessen, Dept Pathol, D-35392 Giessen, Germany Univ Giessen GiessenGermany D-35392 pt Pathol, D-35392 Giessen, Germany
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 1, volume: 280, anno: 2001,
pagine: L58 - L68
SICI:
1040-0605(200101)280:1<L58:MRITAA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; IDIOPATHIC PULMONARY FIBROSIS; CHEMOATTRACTANT PROTEIN-1; IN-VIVO; PERITONEAL-MACROPHAGES; CELLS; RECEPTOR; LUNG; LIPOPOLYSACCHARIDE; INFLAMMATION;
Keywords:
monocyte chemoattractant protein-1; alveolar macrophage; fluorescent dye; flow cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Maus, U Univ Giessen, Dept Internal Med, Klin Str 36, D-35392 Giessen, Germany Univ Giessen Klin Str 36 Giessen Germany D-35392 Giessen, Germany
Citazione:
U. Maus et al., "Monocytes recruited into the alveolar air space of mice show a monocytic phenotype but upregulate CD14", AM J P-LUNG, 280(1), 2001, pp. L58-L68

Abstract

The evaluation of monocytes recruited into the alveolar space under both physiological and inflammatory conditions is hampered by difficulties in discriminating these cells from resident alveolar macrophages (rAMs). Using the intravenous injected fluorescent dye PKH26, which accumulated in rAMs without labeling blood leukocytes, we developed a technique that permits the identification, isolation, and functional analysis of monocytes recruited into lung alveoli of mice. Alveolar deposition of murine JE, the homologue ofhuman monocyte chemoattractant protein (MCP)-1 (JE/MCP-1), in mice provoked an alveolar influx of monocytes that were recovered by bronchoalveolar lavage and separated from PKH26-stained rAMs by flow cytometry. Alveolar recruited monocytes showed a blood monocytic phenotype as assessed by cell surface expression of F4/80, CD11a, CD11b, CD18, CD49d, and CD62L. In contrast,CD14 was markedly upregulated on alveolar recruited monocytes together with increased tumor necrosis factor-alpha message, discriminating this monocyte population from peripheral blood monocytes and rAMs. Thus monocytes recruited into the alveolar air space of mice in response to JE/MCP-1 keep phenotypic features of blood monocytes but upregulate CD14 and are "primed" forenhanced responsiveness to endotoxin with increased cytokine expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:32:54