Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ionizing radiation enhances matrix metalloproteinase-2 production in humanlung epithelial cells
Autore:
Araya, J; Maruyama, M; Sassa, K; Fujita, T; Hayashi, R; Matsui, S; Kashii, T; Yamashita, N; Sugiyama, E; Kobayashi, M;
Indirizzi:
Toyama Med & Pharmaceut Univ, Fac Med, Dept Internal Med 1, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ Toyama Japan 9300194 Toyama 9300194, Japan
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 1, volume: 280, anno: 2001,
pagine: L30 - L38
SICI:
1040-0605(200101)280:1<L30:IREMMP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IDIOPATHIC PULMONARY FIBROSIS; IV COLLAGENASE; TISSUE INHIBITOR; GELATINASE-A; GLUCOCORTICOID RECEPTOR; RAT LUNGS; EXPRESSION; ACTIVATION; P53; PHOSPHORYLATION;
Keywords:
radiation pneumonitis; p53; dexamethasone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Maruyama, M Toyama Med & Pharmaceut Univ, Fac Med, Dept Internal Med 1, 2630 Sugitani,Toyama 9300194, Japan Toyama Med & Pharmaceut Univ 2630 Sugitani Toyama Japan 9300194
Citazione:
J. Araya et al., "Ionizing radiation enhances matrix metalloproteinase-2 production in humanlung epithelial cells", AM J P-LUNG, 280(1), 2001, pp. L30-L38

Abstract

Radiation pneumonitis is a major complication of radiation therapy. However, the detailed cellular mechanisms have not been clearly defined. Based onthe recognition that basement membrane disruption occurs in acute lung injury and that matrix metalloproteinase (MMP)-2 can degrade type IV collagen,one of the major components of the basement membrane, we hypothesized thationizing radiation would modulate MMP-2 production in human lung epithelial cells. To evaluate this, the modulation of MMP-2 with irradiation was investigated in normal human bronchial epithelial cells as well as in A549 cells. We measured the activity of MMP-2 in the conditioned medium with zymography and the MMP-2 mRNA level with RT-PCR. Both of these cells constitutively expressed 72-kDa gelatinolytic activity, corresponding to MMP-2, and exposure to radiation increased this activity. Consistent with the data of zymography, ionizing radiation increased the level of MMP-2 mRNA. This radiation-induced increase in MMP-2 expression was mediated via p53 because the p53 antisense oligonucleotide abolished the increase in MMP-2 activity as well as the accumulation of p53 after irradiation in A549 cells. These resultsindicate that MMP-2 expression by human lung epithelial cells is involved in radiation-induced lung injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 08:54:38