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Titolo:
Inhibition of experimental choroidal neovascularization by overexpression of tissue inhibitor of metalloproteinases-3 in retinal pigment epithelium cells
Autore:
Takahashi, T; Nakamura, T; Hayashi, A; Kamei, M; Nakabayashi, M; Okada, AA; Tomita, N; Kaneda, Y; Tano, Y;
Indirizzi:
Osaka Univ, Sch Med, Dept Ophthalmol, Osaka, Japan Osaka Univ Osaka Japan aka Univ, Sch Med, Dept Ophthalmol, Osaka, Japan Osaka Univ, Sch Med, Div Gene Therapy Sci, Osaka, Japan Osaka Univ OsakaJapan niv, Sch Med, Div Gene Therapy Sci, Osaka, Japan Osaka Univ, Sch Med, Dept Geriatr Med, Osaka 553, Japan Osaka Univ OsakaJapan 553 , Sch Med, Dept Geriatr Med, Osaka 553, Japan
Titolo Testata:
AMERICAN JOURNAL OF OPHTHALMOLOGY
fascicolo: 6, volume: 130, anno: 2000,
pagine: 774 - 781
SICI:
0002-9394(200012)130:6<774:IOECNB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIVO GENE-TRANSFER; EPSTEIN-BARR-VIRUS; MACULAR DEGENERATION; LENTIVIRAL VECTOR; SURGICAL REMOVAL; FUNDUS DYSTROPHY; NUCLEAR-PROTEIN; MURINE RETINA; IN-VITRO; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Takahashi, T Schepens Eye Res Inst, 20 Staniford St,2 W, Boston, MA 02114 USA Schepens Eye Res Inst 20 Staniford St,2 W Boston MA USA 02114
Citazione:
T. Takahashi et al., "Inhibition of experimental choroidal neovascularization by overexpression of tissue inhibitor of metalloproteinases-3 in retinal pigment epithelium cells", AM J OPHTH, 130(6), 2000, pp. 774-781

Abstract

PURPOSE: To evaluate the feasibility of introducing exogenous tissue inhibitor of metalloproteinases-3 gene into the rat retinal pigment epithelium using hemagglutinating virus of Japan liposomes and to assess the effect of tissue inhibitor of metalloproteinases-3 overexpression in retinal pigment epithelium cells on the formation of experimental choroidal neovascularization. METHODS: Hemagglutinating virus of Japan liposomes containing hemagglutin epitope-tagged tissue inhibitor of metalloproreinases-3 gene were injected into the subretinal space in rat eyes. Localization of oligonucleotides wasevaluated by fluorescence microscopy. Exogenous tissue inhibitor of metalloproteinases-3 mRNA expression was assessed by reverse transcribed polymerase chain reaction. Exogenous tissue inhibitor of metalloproteinases-3 protein expression was visualized by immunostaining with monoclonal antibody 12CA5 against the hemagglutin epitope. Three days after transfection of tissueinhibitor of metalloproteinases-3 gene into retinal pigment epithelium cells, intense laser photocoagulation was performed and the incidence of: choroidal neovascularization was assessed by fluorescein fundus angiography. RESULTS: Exogenous tissue inhibitor of metalloproteinases-3 mRNA expression in the choroid and retina was detected on day 3. The efficiency of tissueinhibitor of metalloproteinases-3 gene transfection into retinal pigment epithelium cells was greatest on day 7 and decreased gradually thereafter. The incidence of choroidal neovascularization in tissue inhibitor of metalloproteinases-3 gene-transfected eyes was markedly decreased compared with controls. CONCLUSIONS: This study shows that tissue inhibitor of metaUoproteinases-3gene can be transferred into rat retinal pigment epithelium using the hemagglutinating virus of Japan-liposome method and that tissue inhibitor of metalloproteinases-3 gene overexpression can inhibit development of experimental choroidal neovascularization. This method may represent a future treatment modality for human macular degeneration associated with choroidal neovascularization. (C) 2000 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:02:44