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Titolo:
Vasoactive intestinal peptide influences hatching of ovine blastocysts
Autore:
Leoni, G; Bogliolo, L; Deledda, F; Ledda, S; Naitana, S;
Indirizzi:
Univ Sassari, Dept Anim Biol, I-07100 Sassari, Italy Univ Sassari Sassari Italy I-07100 ept Anim Biol, I-07100 Sassari, Italy
Titolo Testata:
REPRODUCTION FERTILITY AND DEVELOPMENT
fascicolo: 1-2, volume: 12, anno: 2000,
pagine: 7 - 14
SICI:
1031-3613(2000)12:1-2<7:VIPIHO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; EMBRYO DEVELOPMENT INVITRO; FEMALE GENITAL-TRACT; GROWTH-FACTOR; MOUSE EMBRYOS; IN-VITRO; PREIMPLANTATION DEVELOPMENT; AMINO-ACIDS; POLYPEPTIDE VIP; INSULIN;
Keywords:
blastocyst diameter; cell number; plasminogen activator;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Leoni, G Univ Sassari, Dept Anim Biol, V Vienna 2, I-07100 Sassari, Italy Univ Sassari V Vienna 2 Sassari Italy I-07100 100 Sassari, Italy
Citazione:
G. Leoni et al., "Vasoactive intestinal peptide influences hatching of ovine blastocysts", REPROD FERT, 12(1-2), 2000, pp. 7-14

Abstract

Expanded blastocysts collected from superovulated Sarda ewes were divided at random into four groups for culture in a simple medium that does not support blastocyst hatching (CZB) or a complex medium that is permissive to hatching (TCM 199), with or without vasoactive intestinal peptide (VIP), a known embryo mitogenic peptide. Plasminogen activator (PA) secretion after 24h of culture, and the number of cells, diameter of blastocysts and hatching rate after 48 h of culture were compared. The results showed an increase in hatching rate (78.6 v. 6.7%; P<0.01), diameter and number of cells (220.89 v. 210.44 <mu>m, P<0.01 and 246 v. 232, P<0.01 respectively) and caseinolytic areas (1.33 v. 0.92 cm, P<0.01) of blastocysts cultured in TCM 199 compared with those cultured in CZB. Supplementation of the culture media with VIP increased these parameters in CZB (P<0.01) and partially in TCM 199. In particular, cell number, diameter and PA activity were significantly higher (P<0.01) after culture with VIP in both media. Immunoneutralization of exogenous VIP in culture with anti-VIP antibody caused a decrease in the hatching rate (P<0.01) of embryos cultured in medium with VIP, similar to therate in unsupplemented CZB (P<0.01). These results suggest a receptor-mediated response. In immunohistochemical studies, VIP was shown to bind receptors in hatched blastocysts demonstrating the VIP-receptor interaction, and VIP receptors of approximately 150 kDa were revealed by electrophoretic studies. In conclusion, ovine preimplantation embryos exhibit VIP receptors, providing a basis for a receptor-mediated influence of VIP in the hatching of ovine blastocysts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 17:00:39