Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
An aniline blue staining procedure for confocal microscopy and 3D imaging of normal and perturbed cellular phenotypes in mature Arabidopsis embryos
Autore:
Bougourd, S; Marrison, J; Haseloff, J;
Indirizzi:
Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England Univ York York N Yorkshire England YO10 5YW O10 5YW, N Yorkshire, England Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England Univ CambridgeCambridge England CB2 3EA Sci, Cambridge CB2 3EA, England
Titolo Testata:
PLANT JOURNAL
fascicolo: 4, volume: 24, anno: 2000,
pagine: 543 - 550
SICI:
0960-7412(200011)24:4<543:AABSPF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIAL ORGANIZATION; THALIANA ROOT; CALLOSE; GENE;
Keywords:
Arabidopsis thaliana; embryo; confocal microscopy; 3D visualization; aniline blue; cellular architecture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Bougourd, S Univ York, Dept Biol, POB 373, York YO10 5YW, N Yorkshire, England Univ York POB 373 York N Yorkshire England YO10 5YW e, England
Citazione:
S. Bougourd et al., "An aniline blue staining procedure for confocal microscopy and 3D imaging of normal and perturbed cellular phenotypes in mature Arabidopsis embryos", PLANT J, 24(4), 2000, pp. 543-550

Abstract

A new method is described for fluorescent imaging of mature Arabidopsis embryos that enables their cellular architecture to be visualized without theneed for histological sectioning. Mature embryos are stained with aniline blue and cleared with chloral hydrate to allow high-resolution confocal imaging of individual cells within the embryo prior to germination. The technique allows the collection of longitudinal optical sections throughout the cotyledon, hypocotyl and root of wild-type Arabidopsis C24 embryos. Every cell within the mature embryo can be visualized with sufficient clarity and resolution to allow three-dimensional analysis of cellular architecture. Optical sectioning of mutant gnom, short-root and scarecrow embryos, and through root meristems disrupted as a consequence of targeted misexpression of diphtheria toxin, demonstrate the potential of this technique for visualizing the cellular organization of mutant and perturbed embryonic phenotypes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:10:59