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Titolo:
Liposarcoma initiated by FUS/TLS-CHOP: the FUS/TLS domain plays a criticalPole in the pathogenesis of liposarcoma
Autore:
Perez-Losada, J; Sanchez-Martin, M; Rodriguez-Garcia, MA; Perez-Mancera, PA; Pintado, B; Flores, T; Battaner, E; Sanchez-Garcia, I;
Indirizzi:
Univ Salamanca, CSIC, Ctr Invest Canc, Inst Bilogia Mol & Celular Canc, E-37008 Salamanca, Spain Univ Salamanca Salamanca Spain E-37008 ar Canc, E-37008 Salamanca, Spain Ctr Invest & Tecnol, Area Reprod Anim, Madrid 28040, Spain Ctr Invest & Tecnol Madrid Spain 28040 Reprod Anim, Madrid 28040, Spain Univ Salamanca, Serv Anat Patol, Salamanca 37007, Spain Univ Salamanca Salamanca Spain 37007 Anat Patol, Salamanca 37007, Spain Univ Salamanca, Dept Bioquim & Biol Mol, Salamanca 37007, Spain Univ Salamanca Salamanca Spain 37007 & Biol Mol, Salamanca 37007, Spain
Titolo Testata:
ONCOGENE
fascicolo: 52, volume: 19, anno: 2000,
pagine: 6015 - 6022
SICI:
0950-9232(200012)19:52<6015:LIBFTF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MYELOID-LEUKEMIA; RNA-BINDING PROTEIN; CHROMOSOMAL TRANSLOCATION; MYXOID LIPOSARCOMA; TRANSCRIPTIONAL ACTIVATION; ONCOGENIC TRANSFORMATION; CHIMERIC PROTEIN; TLS-CHOP; GENE; FUS;
Keywords:
chromosomal abnormality; malignant solid tumors; adipose tissue; cancer development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Sanchez-Garcia, I Univ Salamanca, CSIC, Ctr Invest Canc, Inst Bilogia Mol & Celular Canc, E-37008 Salamanca, Spain Univ Salamanca Salamanca Spain E-37008 alamanca, Spain
Citazione:
J. Perez-Losada et al., "Liposarcoma initiated by FUS/TLS-CHOP: the FUS/TLS domain plays a criticalPole in the pathogenesis of liposarcoma", ONCOGENE, 19(52), 2000, pp. 6015-6022

Abstract

The most common chromosomal translocation in liposarcomas, t(12;16)(q13;p11), creates the FUS/TLS-CHOP fusion gene. We previously developed a mouse model of liposarcoma by expressing PUS-CHOP in murine mesenchymal stem cells. In order to understand how PUS-CHOP can initiate liposarcoma, we have nowgenerated transgenic mice expressing altered forms of the PUS-CHOP protein. Transgenic mice expressing high levels of CHOP, which lacks the FUS domain, do not develop any tumor despite its tumorigenicity in vitro and widespread activity of the EF1 alpha promoter, These animals consistently show theaccumulation of a glycoprotein material within the terminally differentiated adipocytes, a characteristic figure of liposarcomas associated with PUS-CHOP. On the contrary, transgenic mice expressing the altered form of PUS-CHOP created by the in frame fusion of the PUS domain to the carboxy end of CHOP (CHOP-FUS) developed liposarcomas. No tumors of other tissues were found in these transgenic mice despite widespread activity of the EF1 alpha promoter. The characteristics of the liposarcomas arising in the CHOP-PUS mice were very similar to those previously observed in our PUS-CHOP transgenicmice indicating that the FUS domain is required not only for transformation but also influences the phenotype of the tumor cells. These results provide evidence that the FUS domain of PUS-CHOP plays a specific and critical role in the pathogenesis of liposarcoma.

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Documento generato il 21/09/20 alle ore 06:14:25