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Titolo:
Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos
Autore:
Sharp, DJ; Rogers, GC; Scholey, JM;
Indirizzi:
Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA Univ CalifDavis Davis CA USA 95616 & Cellular Biol, Davis, CA 95616 USA
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 12, volume: 2, anno: 2000,
pagine: 922 - 930
SICI:
1465-7392(200012)2:12<922:CDIRFP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENP-E; CAENORHABDITIS-ELEGANS; RETROGRADE TRANSPORT; CHEMOSENSORY NEURONS; KINETOCHORE MOTOR; DYNACTIN REVEALS; MITOTIC SPINDLES; SEGREGATION; ANAPHASE; KINESIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Scholey, JM Univ Calif Davis, Sect Mol & Cellular Biol, 1 Shields Ave, Davis, CA 95616USA Univ Calif Davis 1 Shields Ave Davis CA USA 95616 CA 95616USA
Citazione:
D.J. Sharp et al., "Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos", NAT CELL BI, 2(12), 2000, pp. 922-930

Abstract

The movement of chromosomes during mitosis occurs on a bipolar, microtubule-based protein machine, the mitotic spindle. It has long been proposed that poleward chromosome movements that occur during prometaphase and anaphaseA are driven by the microtubule motor cytoplasmic dynein, which binds to kinetochores and transports them toward the minus ends of spindle microtubules. Here we evaluate this hypothesis using time-lapse confocal microscopy to visualize, in real time, kinetochore and chromatid movements in living Drosophila embryos in the presence and absence of specific inhibitors of cytoplasmic dynein. Our results show that dynein inhibitors disrupt the alignment of kinetochores on the metaphase spindle equator and also interfere withkinetochore- and chromatid-to-pole movements during anaphase A. Thus, dynein is essential for poleward chromosome motility throughout mitosis in Drosophila embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:52:04