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Titolo:
The Sko1p repressor and Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae
Autore:
Pascual-Ahuir, M; Serrano, R; Proft, M;
Indirizzi:
Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain Univ Politecn Valencia Valencia Spain 46022 antas, Valencia 46022, Spain
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 1, volume: 21, anno: 2001,
pagine: 16 - 25
SICI:
0270-7306(200101)21:1<16:TSRAGA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAP KINASE CASCADE; GENE-EXPRESSION; DNA-BINDING; SIGNAL-TRANSDUCTION; LEUCINE ZIPPER; SALT TOLERANCE; BUDDING YEAST; BZIP PROTEIN; SITE; CREB;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Proft, M Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Camino VeraS-N, Valencia 46022, Spain Univ Politecn Valencia Camino Vera S-N Valencia Spain 46022 pain
Citazione:
M. Pascual-Ahuir et al., "The Sko1p repressor and Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae", MOL CELL B, 21(1), 2001, pp. 16-25

Abstract

In the transcriptional response of Saccharomyces cerevisiae to stress, both activators and repressors are implicated. Here we demonstrate that the ion homeostasis determinant, HAL1, is regulated by two antagonistically operating bZIP transcription factors, the Sko1p repressor and the Gcn4p activator. A single CRE-like sequence (CREHAL1) at position -222 to -215 with the palindromic core sequence TTACGTAA is essential for stress-induced expression of HAL1. Down-regulation of HAL1 under normal growth conditions requires specific binding of Sko1p to CREHAL1 and the corepressor gene SSN6, Releasefrom this repression depends on the function of the high-osmolarity glycerol pathway. The Gcn4p transcriptional activator binds in vitro to the same CREHAL1, and is necessary for up-regulated HAL1 expression in vivo, indicating a dual control mechanism by a repressor-activator pair occupying the same promoter target sequence. gcn4 mutants display a strong sensitivity to elevated K+ or Na+ concentrations in the growth medium. In addition to reduced HAL1 expression, this sensitivity is explained by the fact that amino acid uptake is drastically impaired by high Na+ and K+ concentrations in wild-type yeast cells. The reduced amino acid biosynthesis of gcn4 mutants would result in amino acid deprivation. Together with the induction of HAL1 by amino acid starvation, these results suggest that salt stress and amino acid availability are physiologically interconnected.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 20:13:40