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Titolo:
Adenoviral-mediated uteroglobin gene transfer inhibits neointimal hyperplasia after balloon injury in the rat carotid artery
Autore:
Larson, RA; Naji, M; Lombardi, JV; Naji, A; Koeberlein, B; Golden, MA;
Indirizzi:
Univ Penn, Med Ctr, Harrison Dept Surg Res, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Surg Res, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF VASCULAR SURGERY
fascicolo: 6, volume: 32, anno: 2000,
pagine: 1111 - 1116
SICI:
0741-5214(200012)32:6<1111:AUGTIN>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGESTERONE-BINDING; RABBIT UTEROGLOBIN; PROTEIN CC10; AMINO-ACID; EXPRESSION; CLONING; CELLS; CDNA; PROLIFERATION; MIGRATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Golden, MA Univ Penn, Ctr Med, Dept Surg, 4th Floor,Silverstein,400 SpruceSt, Philadelphia, PA 19104 USA Univ Penn 4th Floor,Silverstein,400 Spruce St Philadelphia PA USA 19104
Citazione:
R.A. Larson et al., "Adenoviral-mediated uteroglobin gene transfer inhibits neointimal hyperplasia after balloon injury in the rat carotid artery", J VASC SURG, 32(6), 2000, pp. 1111-1116

Abstract

Objective: Uteroglobin is a protein with potent anti-inflammatory and immunomodulatory effects. We hypothesize that induction of uteroglobin expression in the artery wall by local adenoviral gene transfer will decrease neointimal hyperplasia in the rat carotid artery after balloon injury. Methods: Seven male Sprague-Dawley rats underwent balloon injury of the common carotid artery. After the injury, with flow occluded, the artery was instilled with 50 muL of the adenoviral vector encoding uteroglobin gene (Ad. UG) at a concentration of 1.35 x 10(11) pfu/mL (n = 7) or 0.68 x 1011 pfu/ml (n = 7) (n = 7), Control animals were similarly treated: either an adenovirus encoding for beta -galactosidase gene (Ad.LacZ) at 1 x 10(11) pfu/mL,(n = 7) or the phosphate-buffered saline (PBS) vehicle (n = 6) was used. The solution was allowed to dwell for 20 minutes. The rats were humanely killed after 14 days by perfusion fixation, and the carotid arteries were sectioned for analysis with computerized planimetry. The intima-media area ratios were calculated for each artery and compared with analysis of variance with Bonferroni/Dunn post hoc testing. One additional rat from the PBS, Ad.LacZ, and Ad.UG (1.35 x 1011 pfu/mL) groups was humanely killed 4 days aftertreatment for carotid artery protein extraction and Western blotting. Results: Uteroglobin protein production was confirmed in the Ad.UG-treatedarteries with Western blotting. Morphometric analysis showed that the Ad.UG group at 1.35 x 1011 pfu/ml had a significantly lower intima-media area ratio than both the Ad.LacZ (P = .002) and PBS (P = .004) controls. The Ad.UG group at 0.68 x 1011 pfu/ml was also significantly different from the Ad.LacZ (P = .003) and PBS (P = .006) controls. There was no statistical difference between the two control groups or between the two Ad.UG groups. Conclusion: Adenoviral gene transfer of uteroglobin, delivered intraluminally after arterial injury causes the production of uteroglobin protein and has an inhibitory effect on neointimal accumulation in the rat model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 12:25:55