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Titolo:
Transport of L-citrulline in neural cell cultures
Autore:
Schmidlin, A; Fischer, S; Wiesinger, H;
Indirizzi:
Univ Tubingen, Inst Physiol Chem, D-72076 Tubingen, Germany Univ TubingenTubingen Germany D-72076 l Chem, D-72076 Tubingen, Germany
Titolo Testata:
DEVELOPMENTAL NEUROSCIENCE
fascicolo: 5-6, volume: 22, anno: 2000,
pagine: 393 - 398
SICI:
0378-5866(200009/12)22:5-6<393:TOLINC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; AMINO-ACID-TRANSPORT; ARGININOSUCCINATE SYNTHETASE; ARGININE TRANSPORT; GLIAL-CELLS; IMMUNOCYTOCHEMICAL EVIDENCE; HYBRID-CELLS; RAT-BRAIN; LOCALIZATION; ASTROCYTES;
Keywords:
amino acid; arginine; glia; neurons; nitric oxide; system L; trafficking;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Wiesinger, H Univ Tubingen, Inst Physiol Chem, Hoppe Seyler Str 4, D-72076Tubingen, Germany Univ Tubingen Hoppe Seyler Str 4 Tubingen Germany D-72076 ny
Citazione:
A. Schmidlin et al., "Transport of L-citrulline in neural cell cultures", DEV NEUROSC, 22(5-6), 2000, pp. 393-398

Abstract

Uptake of L-[C-14]citrulline was studied in cell culture models of the main neural cell populations, in astroglia-rich primary cultures derived from neonatal rat brain, in rat glioma cells C6-BU-1, in cells of the murine microglial clone N11 and in the glioma x neuroblastoma hybrid cell line 108CC15 with neuronal properties. For comparison, cells of the peripheral macrophage cell line RAW 264.7 were also investigated. A saturable component of uptake was found in all cases with KM values between 0.4 and 3.4 mM and V-maxvalues between 15 and 35 nmol min(-1). (mg protein)(-1). A nonsaturable component dominated uptake at high concentrations of extracellular citrulline, Rates of uptake of L-citrulline were not affected when Na+ or Cl- were omitted from the incubation medium or in the presence of depolarizing concentrations of K+. Saturable uptake of citrulline was strongly inhibited by an excess of histidine or beta -2-aminobicyclo(2.2.1)-heptane-2-carboxylic acid; excess amounts of arginine, creatine, glutamate, cysteic acid or N-methyl-craminoisobutyric acid did not reduce citrulline uptake. Preincubation ofthe cells with bacterial lipopolysaccharide and interferon gamma did not stimulate transport of citrulline. The results suggest that at physiologicalconcentrations citrulline is taken up by neural cells with the help of transport system L for large neutral amino acids. Therefore, in the brain, effective utilization of extracellular citrulline as part of an intercellular trafficking of intermediates of an NO/citrulline cycle depends on the concentrations of all neutral amino acids present. Copyright (C) 2000 S. Karger AG, Basel.

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Documento generato il 28/01/21 alle ore 07:59:31