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Titolo:
Identification of urocortin mRNA antisense transcripts in rat tissue
Autore:
Shi, MX; Yan, XL; Ryan, DH; Harris, RBS;
Indirizzi:
Pennington Biomed Res Ctr, Baton Rouge, LA USA Pennington Biomed Res Ctr Baton Rouge LA USA es Ctr, Baton Rouge, LA USA
Titolo Testata:
BRAIN RESEARCH BULLETIN
fascicolo: 3, volume: 53, anno: 2000,
pagine: 317 - 324
SICI:
0361-9230(200010)53:3<317:IOUMAT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICOTROPIN-RELEASING FACTOR; MESSENGER-RNA; NERVOUS-SYSTEM; CRF RECEPTORS; HORMONE GENE; EXPRESSION; NEUROPEPTIDE; PITUITARY; BRAIN; HYPOTHALAMUS;
Keywords:
stress; ribonuclease protection assay; muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Harris, RBS Univ Georgia, Dept Foods & Nutr, Dawson Hall, Athens, GA 30602USA Univ Georgia Dawson Hall Athens GA USA 30602 ens, GA 30602 USA
Citazione:
M.X. Shi et al., "Identification of urocortin mRNA antisense transcripts in rat tissue", BRAIN RES B, 53(3), 2000, pp. 317-324

Abstract

Urocortin (UCN) has 45% sequence homology with corticotropin releasing factor (CRF) and binds to CRF receptors, We used reverse-transcriptase-polymerase chain reaction to demonstrate the presence of UCN RNA in various brain regions and in peripheral tissues. Ribonuclease protection assay (RPA) using sense and antisense riboprobes demonstrated the presence of a naturally occurring antisense UCN RNA transcript in a number of tissues. Northern blotindicated that the antisense transcript was the same size as sense UCN. RPA, using probes that covered bases 1 to 560 of 579 bp sequence of rat UCN, indicated that the antisense sequence was complementary to sense UCN but did not contain an open reading frame. Sense and antisense UCN RNA were co-expressed in all tissues that contained levels of either transcript detectable by RPA, Sense RNA expression was greater than antisense in the midbrain, the two transcripts were expressed equally in the hypothalamus and antisense was expressed at higher levels than sense in the liver, heart, and skeletal muscle. Antisense RNA expression was stress responsive, suggesting that it may play a role in regulating transcription or translation of UCN mRNA. (C) 2000 Elsevier Science Inc.

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Documento generato il 28/11/20 alle ore 00:58:04