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Titolo:
Involvement of oxysterols and lysophosphatidylcholine in the oxidized LDL-induced impairment of serum albumin synthesis by HEPG2 cells
Autore:
Bourdon, E; Loreau, N; Davignon, J; Bernier, L; Blache, D;
Indirizzi:
Univ Bourgogne, Fac Med, INSERM, Biochim Lipoprot & Interact Vasc U498, F-21033 Dijon, France Univ Bourgogne Dijon France F-21033 act Vasc U498, F-21033 Dijon, France Clin Res Inst Montreal, Hyperlipidemia & Atherosclerosis Res Grp, Montreal, PQ H2W 1R7, Canada Clin Res Inst Montreal Montreal PQ Canada H2W 1R7 eal, PQ H2W 1R7, Canada
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 12, volume: 20, anno: 2000,
pagine: 2643 - 2650
SICI:
1079-5642(200012)20:12<2643:IOOALI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; RECEPTOR LXR-ALPHA; FACTOR-KAPPA-B; TRANSCRIPTION FACTOR; ENDOTHELIAL-CELLS; GENE-EXPRESSION; CHOLESTEROL-METABOLISM; OXIDATIVE MODIFICATION; NEPHROTIC SYNDROME; ATHEROSCLEROSIS;
Keywords:
oxidant stress; lipid peroxidation; liver; atherosclerosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Blache, D Univ Bourgogne, Fac Med, INSERM, Biochim Lipoprot & Interact Vasc U498, 7 Bd Jeanne dArc, F-21033 Dijon, France Univ Bourgogne 7 Bd Jeanne dArc Dijon France F-21033 on, France
Citazione:
E. Bourdon et al., "Involvement of oxysterols and lysophosphatidylcholine in the oxidized LDL-induced impairment of serum albumin synthesis by HEPG2 cells", ART THROM V, 20(12), 2000, pp. 2643-2650

Abstract

Oxidized low density lipoproteins (Ox-LDLs) are increasingly thought to bea key element in atherogenesis. We have previously reported that serum albumin has important antioxidant properties and that a reduced synthesis of albumin may represent a crucial point in the overall antioxidant defense. Inthe present work: we aimed at determining whether Ox-LDL could modulate albumin synthesis in cultured human hepatocytes (HepG2 cells). With the use of enzyme immunoassay and radiolabeled leucine incorporation followed by specific immunoprecipitation, Ox-LDL was found to lead to a dose-dependent decrease in albumin secretion. Moreover, the protein synthesis and mRNA levelswere decreased in the presence of Ox-LDL, as assessed by Northern blot analysis. Because oxysterols and lysophospholipids are key components of Ox-LDL, we tested the effects of oxysterols (7-ketocholesterol and 25-hydroxycholesterol) and lysophosphatidylcholine on albumin secretion and expression. In our experimental conditions, we found that incubation's with oxysterols or lysophosphatidylcholine at pathophysiological concentrations similar to those measured in Ox-LDLs reproduced the above-mentioned inhibitory effectson albumin synthesis. On the basis of our in vitro data, we propose that this newly described biological effect of Ox-LDL might partly explain the findings of epidemiological studies indicating that reduced levels of serum albumin are associated with increased mortality.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:33:05