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Titolo:
WESTERN IMMUNOBLOTTING AND ENZYMATIC-ACTIVITY ANALYSIS OF CATHEPSIN-DIN HUMAN BREAST-CANCER CELL-LINES OF DIFFERENT INVASIVE POTENTIAL - REGULATION BY 17-BETA-ESTRADIOL, TAMOXIFEN AND ICI-182,780
Autore:
COUISSI D; DUBOIS V; REMACLE C; SCHONNE E; TROUET A;
Indirizzi:
UNIV CATHOLIQUE LOUVAIN,CELL BIOL LAB,PL CROIX SUD 5 B-1348 LOUVAIN BELGIUM UNIV CATHOLIQUE LOUVAIN,CELL BIOL LAB B-1348 LOUVAIN BELGIUM UNIV CATHOLIQUE LOUVAIN,LAB DEV GENET B-1348 LOUVAIN BELGIUM
Titolo Testata:
Clinical & experimental metastasis
fascicolo: 4, volume: 15, anno: 1997,
pagine: 349 - 360
SICI:
0262-0898(1997)15:4<349:WIAEAO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-BINDING-PROTEINS; PROGNOSTIC VALUE; METASTASIS; ESTROGEN; ANTIESTROGENS; MODULATION; SECRETION; INVITRO; VARIANT; MODEL;
Keywords:
ANTIESTROGENS; BREAST CANCER; CATHEPSIN D; ESTROGENS; INVASIVE POTENTIAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
D. Couissi et al., "WESTERN IMMUNOBLOTTING AND ENZYMATIC-ACTIVITY ANALYSIS OF CATHEPSIN-DIN HUMAN BREAST-CANCER CELL-LINES OF DIFFERENT INVASIVE POTENTIAL - REGULATION BY 17-BETA-ESTRADIOL, TAMOXIFEN AND ICI-182,780", Clinical & experimental metastasis, 15(4), 1997, pp. 349-360

Abstract

We used enzymatic activity and immunochemical quantifications to analyse the expression and secretion of cathepsin D by human breast cancercell lines of different invasive potentials (MCF-7/6, MCF-7/AZ, MDA-MB-231), This study does not directly prove that cathepsin D or procathepsin D is involved in human breast cancer cell invasion and metastasis but it shows that the proportion of procathepsin D (activity and antigen) secreted by the human breast cancer cell lines tested correlateswith their invasive potential. In the estrogen receptor-positive MCF-7 subclones, this proportion is increased by estradiol only in the invasive MCF-7/6 variant, The cell content in procathepsin D is increasedby estrogens to a greater extent in MCF-7/6 cells as compared to non-invasive MCF-7/AZ cells, Tamoxifen appears to be an estrogen agonist concerning cathepsin D regulation, whereas ICI 182,780 is a true antagonist, Our results suggest that synthesis and secretion of cathepsin D are regulated at two distinct levels and differentially affected by estrogens, Synthesis only seems to be affected in non-invasive MCF-7/AZ cells, whereas in invasive MCF-7/6 cells, both synthesis and the efficiency of secretion are increased by estrogens, Our results also confirm that the key site of regulation leading to lysosomal enzyme oversecretion is the Golgi apparatus insulin-like growth factor-II/mannose 6-phosphate receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:00:52