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Titolo:
Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress
Autore:
Azuma, N; Akasaka, N; Kito, H; Ikeda, M; Gahtan, V; Sasajima, T; Sumpio, BE;
Indirizzi:
Yale Univ, Sch Med, Dept Surg, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 Med, Dept Surg, New Haven, CT 06510 USA Asahikawa Med Coll, Dept Surg 1, Asahikawa, Hokkaido 0788510, Japan Asahikawa Med Coll Asahikawa Hokkaido Japan 0788510 kkaido 0788510, Japan
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 1, volume: 280, anno: 2001,
pagine: H189 - H197
SICI:
0363-6135(200101)280:1<H189:ROPMKI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; HEAT-SHOCK PROTEIN-27; SUBSTRATE-SPECIFICITY; SELECTIVE ACTIVATION; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; BLOOD-FLOW; PHOSPHORYLATION; RHO; MODULATION;
Keywords:
mitogen-activated protein kinase; heat shock protein; actin; reorientation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Sumpio, BE Yale Univ, Sch Med, Dept Surg, 333 Cedar St, New Haven, CT 06510 USA Yale Univ 333 Cedar St New Haven CT USA 06510 ven, CT 06510 USA
Citazione:
N. Azuma et al., "Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress", AM J P-HEAR, 280(1), 2001, pp. H189-H197

Abstract

The p38/mitogen-activated protein (MAP) kinase-activated protein kinase 2 (MAPKAP kinase 2)/heat shock protein (HSP)25/27 pathway is thought to play a critical role in actin dynamics. In the present study, we examined whether p38 was involved in the morphological changes seen in endothelial cells (EC) exposed to shear stress. Cultured bovine aortic EC were subjected to 14dyn/cm(2) laminar steady shear stress. Peak activation of p38, MAPKAP kinase 2, and HSP25 were sixfold at 5 min, sixfold at 5 min, and threefold at 30 min compared with static control, respectively. SB-203580 (1 muM), a specific inhibitor of p38, abolished the activation of MAPKAP kinase 2 and HSP25 as well as EC elongation and alignment in the direction of flow elicited by shear stress. The mean orientation angle of cells subjected to shear without SB-203580, with SB-203580, or static control were 17, 50, and 43 degrees, respectively (P< 0.05). EC transfected with the dominant negative mutant of p38-<alpha> aligned randomly with no stress fiber formation despite exposure to shear stress. These data suggests that the pathway of p38/ MAPKAPkinase 2/HSP25/27 is activated in response to shear stress, and this pathway plays an important role in morphological changes induced by shear stress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:13:48