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Titolo:
Activation of MT2 melatonin receptors in rat suprachiasmatic nucleus phaseadvances the circadian clock
Autore:
Hunt, AE; Al-Ghoul, WM; Gillette, MU; Dubocovich, ML;
Indirizzi:
Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 ell & Struct Biol, Urbana, IL 61801 USA Univ Illinois, Neurosci Program, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 Neurosci Program, Urbana, IL 61801 USA Northwestern Univ, Sch Med, Dept Pharmacol & Biol Chem, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 & Biol Chem, Chicago, IL 60611 USA Northwestern Univ, Sch Med, NW Drug Discovery Program, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 ery Program, Chicago, IL 60611 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 1, volume: 280, anno: 2001,
pagine: C110 - C118
SICI:
0363-6143(200101)280:1<C110:AOMMRI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; ACTIVITY RHYTHMS; MESSENGER-RNA; TAIL ARTERY; SUBTYPE; INVITRO; ANTAGONISTS; EXPRESSION; RESPONSES; BRAIN;
Keywords:
suprachiasmatic nucleus; brain slice electrophysiology; luzindole; 4-phenyl-2-propionamidotetraline; protein kinase C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Hunt, AE Univ Illinois, Dept Cell & Struct Biol, B107 CLSL,601 S Goodwin Ave, Urbana, IL 61801 USA Univ Illinois B107 CLSL,601 S Goodwin Ave Urbana IL USA 61801 USA
Citazione:
A.E. Hunt et al., "Activation of MT2 melatonin receptors in rat suprachiasmatic nucleus phaseadvances the circadian clock", AM J P-CELL, 280(1), 2001, pp. C110-C118

Abstract

The aim of this study was to identify the melatonin receptor type(s) (MT1 or MT2) mediating circadian clock resetting by melatonin in the mammalian suprachiasmatic nucleus (SCN). Quantitative receptor autoradiography with 2-[I-125] iodomelatonin and in situ hybridization histochemistry, with eitherP-33- or digoxigenin-labeled antisense MT1 and MT2 melatonin receptor mRNAoligonucleotide probes, revealed specific expression of both melatonin receptor types in the SCN of inbred Long-Evans rats. The melatonin receptor type mediating phase advances of the circadian rhythm of neuronal firing ratein the SCN slice was assessed using competitive melatonin receptor antagonists, the MT1/MT2 nonselective luzindole and the MT2-selective 4-phenyl-2-propionamidotetraline (4P-PDOT). Luzindole and 4P-PDOT (1 nM-1 muM) did not affect circadian phase on their own; however, they blocked both the phase advances (similar to4 h) in the neuronal firing rate induced by melatonin (3pM) at temporally distinct times of day [i.e., subjective dusk, circadian time (CT) 10; and dawn, CT 23], as well as the associated increases in protein kinase C activity. We conclude that melatonin mediates phase advances of the SCN circadian clock at both dusk and dawn via activation of MT2 melatonin receptor signaling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:36:40