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Titolo:
SPECIFIC DETECTION OF MOUSEPOX VIRUS BY POLYMERASE CHAIN-REACTION
Autore:
NEUBAUER H; PFEFFER M; MEYER H;
Indirizzi:
SANITATSAKAD BUNDESWEHR,INST MIKROBIOL,NEUHERBERGSTR 11 D-80937 MUNICH GERMANY FED ARMED FORCES MED ACAD,INST MICROBIOL D-80937 MUNICH GERMANY UNIV MUNICH,INST MED MICROBIOL INFECT & EPIDEM DIS D-80539 MUNICH GERMANY
Titolo Testata:
Laboratory animals
fascicolo: 3, volume: 31, anno: 1997,
pagine: 201 - 205
SICI:
0023-6772(1997)31:3<201:SDOMVB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
INCLUSION PROTEIN; ECTROMELIA VIRUS; COWPOX VIRUS; POXVIRUSES; ANIMALS; BODY;
Keywords:
INFECTIOUS ECTROMELIA; MOUSEPOX VIRUS; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
H. Neubauer et al., "SPECIFIC DETECTION OF MOUSEPOX VIRUS BY POLYMERASE CHAIN-REACTION", Laboratory animals, 31(3), 1997, pp. 201-205

Abstract

Polymerase chain reaction was applied to the rapid identification anddetection of mousepox virus. This was accomplished by selection of primers targeting the A-type inclusion body protein gene. By investigating 20 strains belonging to five different species of the genus Orthopoxvirus, amplification was achieved only with the seven mousepox virus strains examined. The size of the resulting DNA fragment accounted for116 base pairs and contained a recognition site for the restriction enzyme HindII, thus confirming its viral origin. Amplification of mousepox virus specific sequences was also possible from infected mouse lung tissue and serum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:29:49