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Titolo:
Antigenicity and immunogenicity of the HIV-1 gp41 epitope ELDKWA inserted into permissive sites of the MalE protein
Autore:
Coeffier, E; Clement, JM; Cussac, V; Khodaei-Boorane, N; Jehanno, M; Rojas, M; Dridi, A; Latour, M; El Habib, R; Barre-Sinoussi, F; Hofnung, M; Leclerc, C;
Indirizzi:
Inst Pasteur, Unite Biol Regulat Immunitaires, F-75724 Paris 15, France Inst Pasteur Paris France 15 ulat Immunitaires, F-75724 Paris 15, France Inst Pasteur, CNRS, UA 1444, Unite Programmat Mol & Toxicol Genet, Paris, France Inst Pasteur Paris France Programmat Mol & Toxicol Genet, Paris, France Aventis Pasteur, Marcy Etoile, France Aventis Pasteur Marcy Etoile France entis Pasteur, Marcy Etoile, France Inst Pasteur, Unite Biol Retrovirus, Paris, France Inst Pasteur Paris France Pasteur, Unite Biol Retrovirus, Paris, France
Titolo Testata:
VACCINE
fascicolo: 7-8, volume: 19, anno: 2000,
pagine: 684 - 693
SICI:
0264-410X(20001122)19:7-8<684:AAIOTH>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; MALTOSE-BINDING-PROTEIN; B-CELL EPITOPE; HUMAN MONOCLONAL-ANTIBODIES; FUNCTIONAL-CHANGES; NEUTRALIZATION; 2F5; ESCHERICHIA-COLI-K12; IMMUNIZATION; LOCATION;
Keywords:
HIV-1; gp41 epitope; MalE protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Leclerc, C Inst Pasteur, Unite Biol Regulat Immunitaires, 25 Rue Dr Roux, F-75724 Paris 15, France Inst Pasteur 25 Rue Dr Roux Paris France 15 4 Paris 15, France
Citazione:
E. Coeffier et al., "Antigenicity and immunogenicity of the HIV-1 gp41 epitope ELDKWA inserted into permissive sites of the MalE protein", VACCINE, 19(7-8), 2000, pp. 684-693

Abstract

The highly conserved amino acid sequence ELDKWA of HIV-1 gp41 has been inserted into Escherichia coli MalE protein which had been shown to be an adequate carrier to present foreign epitopes to the immune system. We first investigated whether eight different permissive sites of MalE are able to tolerate an insertion of 7-50 residues encoding this epitope. Secondly, antigenicity:of the epitope inserted in MalE protein was estimated from monoclonalantibody 2F5 binding analysis using the BIAcore(R) technology and its immunogenicity in mice was measured as the ability of hybrid proteins to elicitantibodies against a synthetic peptide containing this epitope. This studyrevealed a good correlation between the antigenicity of the inserted epitope and its immunogenicity. Increasing the length of the inserted epitope, as well as inserting multicopies of this epitope increased both its antigenicity and immunogenicity. However, none of the MalE hybrid proteins tested induced anti-HIV-1 neutralizing antibodies. This study strongly suggests that the capacity of the 2F5 epitope to induce neutralizing antibodies dependson the molecular context in which it is presented. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:23:48