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Titolo:
Interaction of Mycobacterium tuberculosis with MH-S, an immortalized murine alveolar macrophage cell line: a comparison with primary murine macrophages
Autore:
Melo, MD; Stokes, RW;
Indirizzi:
Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC V5Z 1M9, Canada Univ British Columbia Vancouver BC Canada V5Z 1M9 ver, BC V5Z 1M9, Canada Univ British Columbia, Dept Pediat, Vancouver, BC V5Z 1M9, Canada Univ British Columbia Vancouver BC Canada V5Z 1M9 ver, BC V5Z 1M9, Canada Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V5Z 1M9, Canada Univ British Columbia Vancouver BC Canada V5Z 1M9 ver, BC V5Z 1M9, Canada British Columbia Childrens Hosp, Div Infect Dis & Immunol, Vancouver, BC V6H 3V4, Canada British Columbia Childrens Hosp Vancouver BC Canada V6H 3V4H 3V4, Canada
Titolo Testata:
TUBERCLE AND LUNG DISEASE
fascicolo: 1, volume: 80, anno: 2000,
pagine: 35 - 46
SICI:
0962-8479(2000)80:1<35:IOMTWM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY; COMPLEMENT RECEPTORS; NONOPSONIC BINDING; MOUSE MACROPHAGE; LEISHMANIA-MAJOR; PHAGOCYTOSIS; ADHESION; CR-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Stokes, RW Res Inst, Dept Pediat, 950 W 28th Ave, Vancouver, BC V5Z 4H4, Canada Res Inst 950 W 28th Ave Vancouver BC Canada V5Z 4H4 4H4, Canada
Citazione:
M.D. Melo e R.W. Stokes, "Interaction of Mycobacterium tuberculosis with MH-S, an immortalized murine alveolar macrophage cell line: a comparison with primary murine macrophages", TUBERC LUNG, 80(1), 2000, pp. 35-46

Abstract

We are interested in identifying a suitable model for investigating mycobacteria interactions with alveolar macrophages. MH-S, a murine alveolar macrophage cell line, is a possible candidate. Objective: To compare the receptor mediated interactions of mycobacteria with primary murine macrophages and MH-S. Design: The association of MH-S monolayers with Mycobacterium tuberculosis(MTB) and other defined particles was compared to that of resident Day 1 peritoneal macrophage (PM) and Day 4 alveolar macrophage (AM) monolayers. Results: In the absence of serum, the association of MTB with MH-S was comparable to that of AM, with approximately 35% of each macrophage type binding at least one bacterium. In contrast, almost 80% of PM bound at least onebacterium. MTB binding was enhanced for all macrophage types by a heat-labile component of normal mouse serum. Antibodies recognising CR3 inhibited the serum-mediated enhanced binding of MTB by MH-S. Binding of latex, immunoglobulin coated or complement coated SRBC by MH-S, AM and PM was comparable. Binding of zymosan by MH-S was greatly inferior to AM and PM. Conclusion: The receptor expression and particle binding properties of MH-S are similar to AM in many, but not all, ways. MH-S, therefore, has the potential to be used as a model for investigating MTB-macrophage interactions. (C) 2000 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 04:43:13