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Titolo:
Polyclonal anticardiolipin antibodies purified from sera of patients with active systemic lupus erythematosus induce apoptosis of the cultured glomerular mesangial cells
Autore:
Tsai, CY; Yu, CL; Wu, TH; Lu, JY; Lair, TS; Tsai, YY; Chou, CT;
Indirizzi:
Natl Yang Ming Univ, Taichung Vet Gen Hosp, Dept Med, Sect Allergy Immunol& Rheumatol, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 nol& Rheumatol, Taipei 112, Taiwan Natl Yang Ming Univ, Taichung Vet Gen Hosp, Dept Med, Nephrol Sect, Taipei112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 d, Nephrol Sect, Taipei112, Taiwan Natl Taiwan Univ Hosp, Dept Med, Taipei, Taiwan Natl Taiwan Univ Hosp Taipei Taiwan Univ Hosp, Dept Med, Taipei, Taiwan
Titolo Testata:
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY
fascicolo: 6, volume: 29, anno: 2000,
pagine: 370 - 379
SICI:
0300-9742(2000)29:6<370:PAAPFS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-DNA ANTIBODIES; ANTIPHOSPHOLIPID ANTIBODIES; IMMUNE-COMPLEXES; C-MYC; NEPHRITIS; MEMBRANE; BINDING; ANTICOAGULANT; PROLIFERATION; CARDIOLIPIN;
Keywords:
anticardiolipin antibodies; apoptosis; glomerular mesangial cell; lupus nephritis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Tsai, CY Natl Yang Ming Univ, Taichung Vet Gen Hosp, Dept Med, Sect Allergy Immunol& Rheumatol, 201 Sect 2,Shih-Pai Rd, Taipei 112, Taiwan Natl Yang Ming Univ 201 Sect 2,Shih-Pai Rd Taipei Taiwan 112 wan
Citazione:
C.Y. Tsai et al., "Polyclonal anticardiolipin antibodies purified from sera of patients with active systemic lupus erythematosus induce apoptosis of the cultured glomerular mesangial cells", SC J RHEUM, 29(6), 2000, pp. 370-379

Abstract

Objective: To test the effect of anticardiolipin antibodies (aCL) on cultured glomerular mesangial cells with regard to their expression of apoptosis-related genes. Methods: aCL purified from active lupus sera by cardiolipin micelles were incubated with cultured rodent mesangial cells (RMC). Morphological changesof the RMC were observed. The genomic DNA was extracted for the detection of apoptosis. The total cell RNA was extracted for detection of Fas, c-myc,p53, and bcl-2 transcripts by reverse transcription-polymerase chain reaction. Results: aCL (100 GPL-U/0.1 mg protein/ml) bound to RMC more prominent than human IgG (100 mug/ml). The antibodies suppressed RMC proliferation in a dose-dependent manner. The RMC were undergoing apoptosis as evidenced by morphologic changes, fluorescein-annexin V staining and appearance of nucleosome-sized DNA fragments. RMC spontaneously express p53 and c-myc but not Fas or bcl-2. aCL (100 GPL-U/ml) enhanced the expression of Fas but not otherapoptosis-related genes and suppressed the intracellular tyrosine phosphorylation. Conclusions: Binding of aCL can induce apoptosis of the RMC. The aCL may be implicated in the pathogenesis of lupus nephritis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:15:44