Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INDUCTION OF MUTATIONS AT THE HYPOXANTHINE PHOSPHORIBOSYL TRANSFERASE(HPRT) LOCUS IN AHH-1 HUMAN LYMPHOBLASTOID-CELLS
Autore:
MORRIS SM; DOMON OE; DELCLOS KB; CHEN JJ; CASCIANO DA;
Indirizzi:
US FDA,NATL CTR TOXICOL RES,DIV GENET TOXICOL JEFFERSON AR 72079 US FDA,NATL CTR TOXICOL RES,DIV BIOCHEM TOXICOL JEFFERSON AR 72079 US FDA,NATL CTR TOXICOL RES,DIV BIOMETRY & RISK ASSESSMENT JEFFERSON AR 72079
Titolo Testata:
Mutation research
fascicolo: 1, volume: 310, anno: 1994,
pagine: 45 - 54
SICI:
0027-5107(1994)310:1<45:IOMATH>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-ADDUCT FORMATION; HAMSTER OVARY CELLS; ISOLATED RAT HEPATOCYTES; ETHYL-N-NITROSOUREA; METABOLIC-ACTIVATION; SALMONELLA-TYPHIMURIUM; GENE MUTATION; 6-NITROCHRYSENE; MUTAGENICITY; 6-AMINOCHRYSENE;
Keywords:
HPRT MUTATIONS; SIMPLE ETHYLATING AGENTS; BENZO[A]PYRENE; 6-NITROCHRYSENE; 6-AMINOCHRYSENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S.M. Morris et al., "INDUCTION OF MUTATIONS AT THE HYPOXANTHINE PHOSPHORIBOSYL TRANSFERASE(HPRT) LOCUS IN AHH-1 HUMAN LYMPHOBLASTOID-CELLS", Mutation research, 310(1), 1994, pp. 45-54

Abstract

Cells from the human lymphoblastoid cell line, AHH-1, were exposed totwo direct-acting mutagens, ethyl methanesulfonate (EMS) and ethyl nitrosourea (ENU), and to three carcinogens that require metabolic activation to an electrophile, benzo[a]pyrene (B(a)P), 6-aminochrysene (6-AC), and 6-nitrochrysene (6-NC); mutation induction at the HPRT locus was quantified by resistance to 6-thioguanine (6-TG(r)). Exposure of AHH-1 cells to either EMS or ENU resulted in a concentration-dependent increase in mutant frequency at the HPRT locus. When AHH-1 cells were exposed to B(a)P, the increase in mutant frequency at the HPRT locus was marginally significant linearly and significant quadratically. The P-32-postlabeling assay revealed the formation of DNA adducts derived from -benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide which may account for the increase in 6-TG(r) clones. Although DNA adducts could be detected by the P-32-postlabeling assay in both 6-NC- and 6-AC-treated AHH-1 cells, exposure to 6-AC or 6-NC did not result in a concentration-dependent increase in mutant frequency at the HPRT locus. Our results are consistent with the results of previous studies which indicate that EMS and ENU are effective inducers of 6-TG(r) clones as is B(a)P when activated to an electrophile. In 6-NC- and 6-AC-exposed cells, lowlevels of N-hydroxy-6-aminochrysene-derived adducts were detected in only 6-NC-exposed cells. No 6-aminochrysene-1,2-dihydrodiol-derived adducts were detected following 6-NC or 6-AC exposure. Minimal metabolicactivation of 6-NC or 6-AC by AHH-1 cells may account for the lack ofa positive mutagenic response for either 6-AC or 6-NC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:38:03