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Titolo:
Chromosome painting reveals specific patterns of chromosome occurrence in mitomycin C- and diethylstilboestrol-induced micronuclei
Autore:
Fauth, E; Scherthan, H; Zankl, H;
Indirizzi:
Univ Kaiserslautern, Abt Humanbiol & Humangenet, D-67653 Kaiserslautern, Germany Univ Kaiserslautern Kaiserslautern Germany D-67653 iserslautern, Germany
Titolo Testata:
MUTAGENESIS
fascicolo: 6, volume: 15, anno: 2000,
pagine: 459 - 467
SICI:
0267-8357(200011)15:6<459:CPRSPO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; RADIATION-INDUCED MICRONUCLEI; CENTROMERIC DNA PROBES; BETA-SATELLITE DNA; HUMAN-LYMPHOCYTES; INSITU HYBRIDIZATION; ACROCENTRIC CHROMOSOMES; 5-AZACYTIDINE TREATMENT; CYTOGENETIC DAMAGE; HUMAN-FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
86
Recensione:
Indirizzi per estratti:
Indirizzo: Fauth, E Univ Kaiserslautern, Abt Humanbiol & Humangenet, Postfach 3049, D-67653 Kaiserslautern, Germany Univ Kaiserslautern Postfach 3049 Kaiserslautern Germany D-67653
Citazione:
E. Fauth et al., "Chromosome painting reveals specific patterns of chromosome occurrence in mitomycin C- and diethylstilboestrol-induced micronuclei", MUTAGENESIS, 15(6), 2000, pp. 459-467

Abstract

Cultures of human blood lymphocytes from three subjects were incubated with the clastogen mitomycin C (MMC, 500 ng/ml) and the aneugen diethylstilboestrol (DES, 80 muM) 23 h before harvesting, to induce formation of micronuclei (MN) and numerical and structural alterations in metaphase chromosomes. We used fluorescence in situ hybridization (FISH) with painting probes forall human chromosomes to determine which chromosomes had contributed material to the induced MN. MMC treatment induced an similar to 18-fold increasein MN and led to a significant increase in hypodiploidy and structural chromosome aberrations in metaphase preparations. Undercondensation of pericentromeric heterochromatin of chromosomes 9 and 1 occurred in 20-75% of metaphases and FISH disclosed an abundance of material from these chromosomes ininduced MN (62-69% from chromosome 9 and 7-12% from chromosome 1), DES treatment of lymphocytes induced a seven-fold increase in MN frequency and four-fold increase in the frequency of numerical aberrations; structural aberrations were not significantly increased. FISH analysis showed that materialfrom all chromosomes was present in DES-induced MN, with material from chromosome 1 present in 16% of MN and material from each other chromosomes being present in 2-10% of MN. Material from chromosomes 14, 19 and 21 was significantly more frequent material from chromosome Y significantly less frequent in DES-treated cells than in controls. The findings of the MMC studies indicate that the heterochromatin block of chromosome 9 is a specific target for MMC-induced undercondensation, which induces a preferential occurrence of chromosome 9 material in MN. DES, in contrast, does not trigger heterochromatin decondensation and fails to induce such a significant appearance of material of particular chromosomes in MN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:17:18