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Titolo:
Novel sperm-binding proteins of epididymal origin contain four fibronectintype II-modules
Autore:
Saalmann, A; Munz, S; Ellerbrock, K; Ivell, R; Kirchhoff, C;
Indirizzi:
Univ Hamburg, IHF, Inst Hormone & Fertil Res, D-22529 Hamburg, Germany Univ Hamburg Hamburg Germany D-22529 ertil Res, D-22529 Hamburg, Germany
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 58, anno: 2001,
pagine: 88 - 100
SICI:
1040-452X(200101)58:1<88:NSPOEO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE SEMINAL PLASMA; GENE-EXPRESSION; MAJOR PROTEINS; DOG EPIDIDYMIS; GELATIN-BINDING; MESSENGER-RNA; LIPID EFFLUX; SPERMATOZOA; CAPACITATION; HEPARIN;
Keywords:
human, canine, and equine cDNA sequences; in situ hybridization; Northern and Western blot analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kirchhoff, C Univ Hamburg, IHF, Inst Hormone & Fertil Res, Grandweg 64, D-22529 Hamburg, Germany Univ Hamburg Grandweg 64 Hamburg Germany D-22529 rg, Germany
Citazione:
A. Saalmann et al., "Novel sperm-binding proteins of epididymal origin contain four fibronectintype II-modules", MOL REPROD, 58(1), 2001, pp. 88-100

Abstract

Novel fibronectin type II (Fn2)module proteins were cloned from human and canine epididymal cDNA libraries. cDNA sequences predicted a highly conserved protein family, related but not homologous to ungulate seminal plasma proteins (approximately 50% sequence identity), and the first known examples of proteins with four tandemly arranged Fn2-domains. By Northern blot and in situ hybridization analyses the encoding mRNAs were shown to be abundant products of the epididymal duct epithelium, but not detectable in other tissues. Homologous mRNAs were identified in the epididymides of various mammals, representing members of this novel protein family of epididymal origin. Within the Fn2-module-encoding stretches, species homologues displayed >85% sequence identity, but showed high variability at their predicted N-termini. An antipeptide antiserum in Western blot analyses detected 30-35 kDa immunoreactive protein bands in epididymal tissue, cauda epididymidal fluid, and sperm membrane protein preparations. The tandem arrangement of increasing numbers of Fn2-modules might functionally correspond to the tendency to form oligomers that has been described for lipid-binding proteins. (C) 2001Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:48:56