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Titolo:
Expression of Cu/Zn and Mn superoxide dismutases during bovine embryo development: Influence of in vitro culture
Autore:
Lequarre, AS; Feugang, JM; Malhomme, O; Donnay, I; Massip, A; Dessy, F; Van Langendonckt, A;
Indirizzi:
Univ Catholique Louvain, Unite Sci Vet, B-1348 Louvain, Belgium Univ Catholique Louvain Louvain Belgium B-1348 , B-1348 Louvain, Belgium
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 58, anno: 2001,
pagine: 45 - 53
SICI:
1040-452X(200101)58:1<45:EOCAMS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENCODING ANTIOXIDANT ENZYMES; IN-VITRO; MOUSE EMBRYOS; PREIMPLANTATION EMBRYOS; RELATIVE ABUNDANCE; MAMMALIAN EMBRYOS; GENE TRANSCRIPTS; SINGLE OOCYTES; INVITRO; SERUM;
Keywords:
RT-PCR; bovine oocyte; preimplantation; development; antioxidant enzymes; in vitro/in vivo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Lequarre, AS Univ Catholique Louvain, Unite Sci Vet, Pl Croix Sud 3, B-1348 Louvain, Belgium Univ Catholique Louvain Pl Croix Sud 3 Louvain Belgium B-1348
Citazione:
A.S. Lequarre et al., "Expression of Cu/Zn and Mn superoxide dismutases during bovine embryo development: Influence of in vitro culture", MOL REPROD, 58(1), 2001, pp. 45-53

Abstract

Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase-polymerase chain reaction (RTPCR) and, at a protein level, by Western blotting on pools of embryos. mRNA encoding Cu/Zn SOD were detectedin in vitro bovine embryos throughout preattachment development as well asin in vivo derived morulae and blastocysts. Transcripts for Mn SOD gene were detected in most immature and in vitro matured oocytes as well as in some zygotes and 5- to 8-cell embryos while no transcript was found at the 9- to 16-cell stage in both culture conditions. In vitro embryonic expression of Mn SOD was detected earlier in the presence of serum. Half of the morulae showed the transcript if cultured with 5% serum while none without serum. At the blastocyst stage Mn SOD could be detected independently of culture conditions. For in vivo-derived embryos Mn SOD,transcripts were detected both in morulae and blastocysts. Immunoblotting analyses revealed that Cu/Zn SOD and Mn SOD were also present at a protein level in in vitro-derived zygotes and blastocysts. Together these data demonstrate, for the first time, that Mn SOD is transcribed and that Cu/Zn and Mn SOD proteins are expressedin preimplantation bovine embryos. Finally, they suggest that Mn SOD transcription is altered by in vitro culture conditions. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:52:20