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Titolo:
Integrin expression and IgA nephropathy: In vitro modulation by IgA with altered glycosylation and macromolecular IgA
Autore:
Peruzzi, L; Amore, A; Cirina, P; Trusolino, L; Basso, G; Ricotti, E; Emancipator, SN; Marchisio, PC; Coppo, R;
Indirizzi:
Regina Margherita Childrens Hosp, Nephrol & Dialysis Dept, I-10126 Turin, Italy Regina Margherita Childrens Hosp Turin Italy I-10126 -10126 Turin, Italy San Raffaele Sci Inst, Dept Biol & Technol Res, I-20132 Milan, Italy San Raffaele Sci Inst Milan Italy I-20132 hnol Res, I-20132 Milan, Italy Univ Turin, Dept Biomed Sci & Human Oncol, I-10124 Turin, Italy Univ Turin Turin Italy I-10124 d Sci & Human Oncol, I-10124 Turin, Italy Univ Turin, Pediat Clin, I-10124 Turin, Italy Univ Turin Turin Italy I-10124 Turin, Pediat Clin, I-10124 Turin, Italy Case Western Reserve Univ, Inst Pathol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 6, volume: 58, anno: 2000,
pagine: 2331 - 2340
SICI:
0085-2538(200012)58:6<2331:IEAINI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOGLOBULIN-A NEPHROPATHY; EXTRACELLULAR-MATRIX PROTEINS; HUMAN MESANGIAL CELLS; GROWTH-FACTOR-BETA; ADHESION; FIBRONECTIN; RECEPTORS; BINDING; PROLIFERATION; ASSOCIATION;
Keywords:
degalactosylated IgA; apoptosis; cell-matrix interaction; extracellular matrix; transmembrane proteins; mesangial matrix; glomerular hemodynamics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Coppo, R Regina Margherita Childrens Hosp, Nephrol & Dialysis Dept, PiazzaPolonia 94, I-10126 Turin, Italy Regina Margherita Childrens Hosp Piazza Polonia 94 Turin Italy I-10126
Citazione:
L. Peruzzi et al., "Integrin expression and IgA nephropathy: In vitro modulation by IgA with altered glycosylation and macromolecular IgA", KIDNEY INT, 58(6), 2000, pp. 2331-2340

Abstract

Background. Signal transduction by mesangial cell (MC) integrins regulatescell growth and survival, extracellular matrix production, and organization. The aim of the study was to investigate human MC integrin modulation by differently glycosylated IgA and macromolecular IgA, which are thought to play a pathogenetic role in IgA nephropathy (IgAN). Methods. MCs were incubated with purified human polymeric IgA, heat-aggregated IgA, IgA glycoforms generated by enzymatic hydrolysis of saccharide residues and serum fractions from IgAN patients, and controls isolated by lectin affinity and containing IgA with peculiar glycan patterns. Integrins were quantitated by flow cytometry. Results. Cultured MCs highly expressed alpha (v)beta (3) and some alpha (3)beta (1); alpha (v)beta (3) was up-regulated by matrix components (P < 0.02). In vitro desialylated and degalactosylated polymeric human IgA enhanced<alpha>(v)beta (3) expression on cultured MCs (P < 0.001). Serum IgA glycoforms isolated from IgAN patients with high exposure of internal sugars, GalNAc, Neu5Ac2,6GalNAc, and Man enhanced a, expression on cultured MCs more than healthy controls. Conclusions. These data support the hypothesis that IgA glycation plays a role in modulating the cell-matrix interaction, and that this mechanism canbe operating in IgAN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 10:27:09