Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DNA extraction from coral reef sediment bacteria for the polymerase chain reaction
Autore:
Guthrie, JN; Moriarity, DJW; Blackall, LL;
Indirizzi:
Univ Queensland, Dept Microbiol, Ctr Bacterial Divers & Identificat, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia Acuabiotech LLC, Brisbane, Qld 4160, Australia Acuabiotech LLC Brisbane Qld Australia 4160 Brisbane, Qld 4160, Australia
Titolo Testata:
JOURNAL OF MICROBIOLOGICAL METHODS
fascicolo: 2, volume: 43, anno: 2000,
pagine: 73 - 80
SICI:
0167-7012(200012)43:2<73:DEFCRS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA; REACTION AMPLIFICATION; RAPID EXTRACTION; MICROBIAL DNA; SOIL; DIVERSITY; COMMUNITY; SAMPLES;
Keywords:
ARDRA; bacteria; coral reef sediments; DNA extraction; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Guthrie, JN Univ Cent Queensland, Plant Sci Grp, Rockhampton, Qld 4702, Australia Univ Cent Queensland Rockhampton Qld Australia 4702 Australia
Citazione:
J.N. Guthrie et al., "DNA extraction from coral reef sediment bacteria for the polymerase chain reaction", J MICROB M, 43(2), 2000, pp. 73-80

Abstract

A rapid and effective method for the direct extraction of high molecular weight amplifiable DNA from two coral reef sediments was developed. DNA was amplified by the polymerase chain reaction (PCR) using 16S rDNA specific primers. The amplicons were digested with HaeIII, HinP1I and MspI and separated using polyacrylamide gel electrophoresis and silver staining. The resulting amplified ribosomal DNA restriction analysis (ARDRA) patterns were usedas a fingerprint to discern differences between the coral reef sediment samples. Results indicated that ARDRA is an effective method for determining differences within the bacterial community amongst different environmental samples. (C) 2000 Published by Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 06:51:56