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Titolo:
Effects of initial dose on eastern equine encephalomyelitis virus dependent mortality in intrathoracically inoculated Culiseta melanura (Diptera : culicidae)
Autore:
Cooper, LA; Sina, BJ; Turell, MJ; Scott, TW;
Indirizzi:
Univ Maryland, Dept Entomol, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 t Entomol, College Pk, MD 20742 USA
Titolo Testata:
JOURNAL OF MEDICAL ENTOMOLOGY
fascicolo: 6, volume: 37, anno: 2000,
pagine: 815 - 819
SICI:
0022-2585(200011)37:6<815:EOIDOE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
VALLEY FEVER VIRUS; ENCEPHALITIS-VIRUS; MOSQUITO VECTOR; INFECTION; MIDGUT;
Keywords:
Culiseta melanura; mosquito mortality; arbovirus infection; eastern equine encephalomyelitis virus; virulence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Cooper, LA Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 ge, LA 70803 USA
Citazione:
L.A. Cooper et al., "Effects of initial dose on eastern equine encephalomyelitis virus dependent mortality in intrathoracically inoculated Culiseta melanura (Diptera : culicidae)", J MED ENT, 37(6), 2000, pp. 815-819

Abstract

Viral growth characteristics that favor rapid and prodigious virion production may increase virus transmission but be detrimental to infected hosts. Several arboviruses, including eastern equine encephalomyelitis (EEE) virus, negatively affect the survival of their infected mosquito vectors. To test the hypothesis that the mosquito virulent properties of EEE virus are caused by the presence of intrinsic viral growth properties, we investigated the effects of infecting dose on the survival of intrathoracically inoculated Culiseta melanura (Coquillett). Daily survival of age-matched females inoculated with either a low initial dose of 10(1.5) plaque-forming units (PFUs) per mosquito or a high initial dose of 10(5.5) PFUs per mosquito was monitored for 8 wk. Compared with diluent inoculated controls, mosquitoes fromboth dosage groups displayed highly significant decreases in survival. No significant differences in daily survival were detected between the two infected groups. Virus production within inoculated mosquitoes was assessed bysampling mosquitoes every 12 h for 96 h after inoculation. Rapid virus amplification occurred in both dosage groups, and by 24 h after exposure the mean viral loads in mosquitoes inoculated with the low dose were comparable to those inoculated with the high dose. Likewise, although detectable virions appeared sooner in the saliva of high dosage mosquitoes, by 72 h after inoculation no significant differences in virus transmission were detected between the two exposure groups. These results indicate that the virulence of EEE virus for its enzootic North American mosquito vector is not dosage dependent and likely reflects the inherent growth propel-ties of this virus within infected mosquitoes.

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Documento generato il 03/04/20 alle ore 19:25:15