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Titolo:
Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii
Autore:
Lee, CH; Lasbury, ME; Paulsrud, JR; Bauer, NL; Brady, SL; Weinberg, GA; Durant, PJ; Bartlett, MS; Smith, JW;
Indirizzi:
Indiana Univ, Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ab Med, Indianapolis, IN 46202 USA Univ Rochester, Sch Med & Dent, Dept Pediat, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 ept Pediat, Rochester, NY 14642 USA
Titolo Testata:
JOURNAL OF EUKARYOTIC MICROBIOLOGY
fascicolo: 6, volume: 47, anno: 2000,
pagine: 581 - 584
SICI:
1066-5234(200011/12)47:6<581:COTGEA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR SURFACE GLYCOPROTEIN; BRADYRHIZOBIUM-JAPONICUM; BINDING PROTEIN; CLEAVAGE SITES; HYDROGENASE; NICKEL; HYPB; PREDICTION; DIVERSITY; DATABASE;
Keywords:
AIDS; glycoprotein A; immunosuppression; major surface glycoprotein; nickel-binding protein; opportunistic pathogen; pulsed-field gel electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, CH Indiana Univ, Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 Indianapolis, IN 46202 USA
Citazione:
C.H. Lee et al., "Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii", J EUKAR MIC, 47(6), 2000, pp. 581-584

Abstract

A cDNA clone derived from Pneumocystis carinii contained an unusual sequence (GTGATG)(2)(ATGGTG)(4)(ATG)(4) and many GAT repeats. It was found to encode a histidine and aspartic acid-rich protein (HARP). The complete cDNA contained an 888-bp open reading frame encoding a putative protein of 32.6 kDa. The deduced HARP protein contained 39 aspartic acid and 22 histidine residues. The genomic copy of the HARP gene (1203 bp in length) was found to contain 3 small introns of 46, 44, and 38 bp, respectively. HARP was predicted by computer programs to be a plasma membrane protein with nickel-bindingactivity.

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Documento generato il 14/07/20 alle ore 08:19:14