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Titolo:
Synergistic inhibition of HIV-1 in activated and resting peripheral blood mononuclear cells, monocyte-derived macrophages, and selected drug-resistant isolates with nucleoside analogues combined with a natural product, resveratrol
Autore:
Heredia, A; Davis, C; Redfield, R;
Indirizzi:
Univ Maryland, Inst Biotechnol, Inst Human Virol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Human Virol, Baltimore, MD 21201 USA Univ Maryland Med, Baltimore, MD USA Univ Maryland Med Baltimore MD USAUniv Maryland Med, Baltimore, MD USA Univ Maryland, Dept Med, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 d, Dept Med, Baltimore, MD 21201 USA Univ Maryland, Dept Microbiol & Immunol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 l & Immunol, Baltimore, MD 21201 USA
Titolo Testata:
JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
fascicolo: 3, volume: 25, anno: 2000,
pagine: 246 - 255
SICI:
1525-4135(20001101)25:3<246:SIOHIA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; PROTEIN-KINASE-C; IN-VITRO; REPLICATION; HYDROXYUREA; LYMPHOCYTES; DIDANOSINE; ENZYMES; AIDS; DNA;
Keywords:
resveratrol; HIV-1; hydroxyurea; didanosine; resting lymphocytes; antiproliferation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Redfield, R Univ Maryland, Inst Biotechnol, Inst Human Virol, 725 W Lombard St, Baltimore, MD 21201 USA Univ Maryland 725 W Lombard St Baltimore MD USA 21201 1201 USA
Citazione:
A. Heredia et al., "Synergistic inhibition of HIV-1 in activated and resting peripheral blood mononuclear cells, monocyte-derived macrophages, and selected drug-resistant isolates with nucleoside analogues combined with a natural product, resveratrol", J ACQ IMM D, 25(3), 2000, pp. 246-255

Abstract

Resveratrol (trans-3,5,4'-trihydroxystilbene) is a phytoalexin present in grapes, wine, and certain plants, which has recently been reported to possess properties that may protect against atherosclerosis, certain cancers, and inflammation. We now report that resveratrol (RV) synergistically enhances the anti-HIV-1 activity of the nucleoside analogues zidovudine (AZT), zalcitabine (ddC), and didanosine (ddI). RV at 10 muM was not toxic to cells, and by itself reduced viral replication by 20% to 30%. In phytohemagglutinin (PHA)-activated peripheral blood mononuclear cells (PBMCs) infected with HTLV-IIIB, 10 muM RV reduced the 90 % inhibitory concentration (IC,,) of AZT, ddC, and ddI by 3.5-, 5.5-, and 17.8-fold, respectively. Similar antiviral activity was demonstrated when ddI was combined with 5 or 10 mM RV in PBMCs infected with clinical isolates of HIV-1. The addition of RV resulted in a >10-fold augmentation of ddI-antiviral activity in infected monocyte-derived macrophages (MDMs). In a resting cell model of T lymphocytes which were infected with HTLV-IIIB, RV plus ddI in combination, but not individually, suppressed establishment of a productive viral infection. In addition, RV plus ddI markedly inhibited the replication of four ddI-resistant viral isolates, three of which presented mutations in the RT gene conferring RT-multidrug resistance. Finally, when compared with hydroxyurea (HU), both 100 mM HU and TO mM RV showed similar enhancement of ddI-antiviral suppressive activity. However, RV was shown to have less of a cellular antiproliferative effect than HU.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 23:03:22