Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Antigen-specific proliferation of porcine CD8 alpha alpha cells to an extracellular bacterial pathogen
Autore:
Waters, WR; Hontecillas, R; Sacco, RE; Zuckermann, FA; Harkins, KR; Bassaganya-Riera, J; Wannemuehler, MJ;
Indirizzi:
Iowa State Univ, Vet Med Res Inst, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 v, Vet Med Res Inst, Ames, IA 50011 USA ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA ARS Ames IA USAARS, Natl Anim Dis Ctr, USDA, Ames, IA USA Univ Illinois, Coll Vet Med, Dept Vet Pathobiol, Urbana, IL 61801 USA UnivIllinois Urbana IL USA 61801 ept Vet Pathobiol, Urbana, IL 61801 USA Iowa State Univ, Flow Cytometry Facil, Ames, IA USA Iowa State Univ Ames IA USA ate Univ, Flow Cytometry Facil, Ames, IA USA Iowa State Univ, Dept Anim Sci, Ames, IA USA Iowa State Univ Ames IA USAIowa State Univ, Dept Anim Sci, Ames, IA USA
Titolo Testata:
IMMUNOLOGY
fascicolo: 3, volume: 101, anno: 2000,
pagine: 333 - 341
SICI:
0019-2805(200011)101:3<333:APOPCA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERPULINA-HYODYSENTERIAE BACTERIN; LISTERIA-MONOCYTOGENES INFECTION; KNOCK-OUT MICE; CD8(+) T-CELLS; INTRACELLULAR BACTERIA; INTRAEPITHELIAL LYMPHOCYTES; FUNCTIONAL-CHARACTERIZATION; MONOCLONAL-ANTIBODIES; IMMUNE-RESPONSES; GAMMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Waters, WR Iowa State Univ, Vet Med Res Inst, 1802 Elwood Dr, Ames, IA 50011 USA Iowa State Univ 1802 Elwood Dr Ames IA USA 50011 , IA 50011 USA
Citazione:
W.R. Waters et al., "Antigen-specific proliferation of porcine CD8 alpha alpha cells to an extracellular bacterial pathogen", IMMUNOLOGY, 101(3), 2000, pp. 333-341

Abstract

A vaccine inducing protective immunity to a spirochaete-induced colitis ofpigs predominantly stimulates expansion of CD8(+) cells in vivo and in antigen-stimulated lymphocyte cultures. CD8(+) cells, however, are rarely considered necessary for protection against extracellular bacterial pathogens. In the present study, pigs recovering from colitis resulting from experimental infection with Brachyspira (Serpulina) hyodysenteriae had increased percentages of peripheral blood CD4(-) CD8(+) (alpha alpha -expressing) cells compared with non-infected pigs. CD8 alpha alpha (+) cells proliferated in antigen-stimulated cultures of peripheral blood mononuclear cells from B. hyodysenteriae-vaccinated pigs. Proliferating CD8 alpha alpha (+) cells consisted of CD4(-), CD4(+) and gamma delta T-cell receptor-positive cells. CD4(-) CD8 alpha beta (+) cells from vaccinated or infected pigs did not proliferate upon in vitro antigen stimulation. Of the CD8 alpha alpha cells thathad proliferated, flow cytometric analysis indicated that the majority of the CD4(+) CD8(+) cells were large (i.e. lymphoblasts) whereas the CD4(-) CD8(+) cells were predominantly small. Addition of monoclonal antibodies (mAb) specific for either porcine major histocompatibility complex (MHC) classI or class II antigens diminished B. hyodysenteriae-specific proliferativeresponses whereas addition of mAb to porcine MHC II, but not porcine MHC I, reduced the CD8 alpha alpha response. In vitro depletion of CD4(+) cells by flow cytometric cell sorting diminished, but did not completely abrogate, the proliferative response of cells from vaccinated pigs to B. hyodysenteriae antigen stimulation. These results suggest that CD8 alpha alpha cells are involved in recovery and possibly protection from a spirochaete-inducedcolitis of pigs; yet, this response appears to be partially dependent uponCD4(+) cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 11:44:10