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Titolo:
Rab11 is associated with GLUT4-containing vesicles and redistributes in response to insulin
Autore:
Kessler, A; Tomas, E; Immler, D; Meyer, HE; Zorzano, A; Eckel, J;
Indirizzi:
Diabet Res Inst, D-40225 Dusseldorf, Germany Diabet Res Inst Dusseldorf Germany D-40225 , D-40225 Dusseldorf, Germany Univ Barcelona, Dept Biochem & Mol Biol, Barcelona, Spain Univ Barcelona Barcelona Spain ept Biochem & Mol Biol, Barcelona, Spain Ruhr Univ Bochum, Inst Physiol Chem, D-4630 Bochum, Germany Ruhr Univ Bochum Bochum Germany D-4630 siol Chem, D-4630 Bochum, Germany
Titolo Testata:
DIABETOLOGIA
fascicolo: 12, volume: 43, anno: 2000,
pagine: 1518 - 1527
SICI:
0012-186X(200012)43:12<1518:RIAWGV>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-TRANSPORTER GLUT4; GTP-BINDING PROTEINS; AMINO-ACID-SEQUENCES; RAT ADIPOSE-CELLS; CARDIAC-MUSCLE; INDUCED TRANSLOCATION; MOLECULAR-WEIGHT; PLASMA-MEMBRANE; SKELETAL-MUSCLE; MAJOR PROTEIN;
Keywords:
GLUT4 trafficking; Rab11; cardiac muscle; GLUT4-containing vesicles; small GTP-binding proteins; Zucker rats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Eckel, J Diabet Res Inst, Hennekamp 65, D-40225 Dusseldorf, Germany DiabetRes Inst Hennekamp 65 Dusseldorf Germany D-40225 Germany
Citazione:
A. Kessler et al., "Rab11 is associated with GLUT4-containing vesicles and redistributes in response to insulin", DIABETOLOG, 43(12), 2000, pp. 1518-1527

Abstract

Aims/hypothesis. To identify a GTPase of 24 000 M-r which we recently found to co-localize with GLUT4 in cardiac muscle. Methods. A 24 000 M-r-GTP-binding fraction was purified from pig heart by a three-step chromatographic procedure, followed by two-dimensional electrophoresis and electrospray ionization-mass spectrometry, Subcellular distribution of the GTPase was assessed by western blotting. Go-localization with GLUT4 was assessed by continuous sucrose density gradient fractionation andimmunoadsorption of GLUT4-containing vesicles. Results. The Rab11 protein was identified as a major component of the GTP-binding fraction and its expression in rat cardiac muscle was confirmed. Invivo insulin treatment resulted in the recruitment of Rab11 from the microsomal fraction to the plasma membrane. Subcellular fractionation indicated two immunoreactive GLUT4 pools. Most of the intracellular pool of Rab11 overlapped with the high-density GLUT4 pool and most of the transferrin receptor pool. The Rab11 protein also co-sedimented with the low-density, non-endosomal GLUT4 pool and substantially increased in this fraction after insulin treatment. It was specifically present in GLUT4-containing vesicles and insulin increased its abundance in these vesicles 2.2-fold relative to the amount of GLUT4. These vesicles also containend Rab4 and Akt-2, the latter being only associated after insulin stimulation. Insulin was unable to alterthe cellular localization of Rab11 in insulin-resistant obese Zucker rats. Conclusion/interpretation. These results support the hypothesis that at least two GTPases of the Rab family participate in GLUT4-vesicle trafficking. We suggest that Rab11 is involved in the endosomal recycling, sorting and exocytotic movement of the glucose transporter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 10:30:34