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Titolo:
Determination of the designer drugs 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC and fluorescence detection in whole blood, serum, vitreous humor, and urine
Autore:
Clauwaert, KM; Van Bocxlaer, JF; De Letter, EA; Van Calenbergh, S; Lambert, WE; De Leenheer, AP;
Indirizzi:
State Univ Ghent, Toxicol Lab, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 Toxicol Lab, B-9000 Ghent, Belgium State Univ Ghent, Med Chem Lab, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 ed Chem Lab, B-9000 Ghent, Belgium State Univ Ghent, Lab Forens Med, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 Forens Med, B-9000 Ghent, Belgium
Titolo Testata:
CLINICAL CHEMISTRY
fascicolo: 12, volume: 46, anno: 2000,
pagine: 1968 - 1977
SICI:
0009-9147(200012)46:12<1968:DOTDD3>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMATOGRAPHY MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; MDMA ECSTASY; AMPHETAMINE; METABOLITES; METHAMPHETAMINE; IDENTIFICATION; DERIVATIVES; ANALOGS; PLASMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: De Leenheer, AP State Univ Ghent, Toxicol Lab, Harelbekestr 72, B-9000 Ghent, Belgium State Univ Ghent Harelbekestr 72 Ghent Belgium B-9000 ium
Citazione:
K.M. Clauwaert et al., "Determination of the designer drugs 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC and fluorescence detection in whole blood, serum, vitreous humor, and urine", CLIN CHEM, 46(12), 2000, pp. 1968-1977

Abstract

Background: The popular designer drugs 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyethylamphetamine (MDEA) can be determined in serum, whole blood, and urine, but also in vitreous humor. The latter matrix is interesting when dealing with decomposed bodies in a toxicological setting. Methods: After extraction, chromatographic separation was achieved on a narrow-bore C-18 column by gradient elution with fluorometric detection; results were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: The method was linear over the range of 2-1000 mug/L for whole blood, serum, and vitreous humor, and 0.1-5 mg/L for urine. Extraction recoveries were >70%, imprecision (CV) was 2.5-19%, and analytical recoveries were 95.5-104.4%. The limit of detection (LOD) and the limit of quantification(LOQ) were 0.8 and 2 mug/L, respectively, for whole blood, serum, and vitreous humor, and 2.5 mug/L and 0.1 mg/L, respectively, for urine. Excellent correlations between the quantitative LC-fluorescence and LC-MS/MS results were obtained. We found the following concentrations in a thanatochemical distribution study in rabbits: in serum, 5.3-685 mug/L for MDMA and from theLOQ to 14.5 mug/L for 3,4-methylenedioxyamphetamine (MDA); in whole blood,19.7-710 mug/L for MDMA and from the LOQ to 17.8 mug/L for MDA; in vitreous humor, 12.1-97.8 mug/L for MDMA and from the LOQ to 3.86 mug/L for MDA. In routine toxicological urine samples, concentrations ranged from LOQ to 14,62 mg/L for MDA, from LOQ to 157 mg/L for MDMA, and from LOQ to 32.54 mg/Lfor MDEA. Conclusions: The HPLC method described is sensitive, specific, and suitable for the determination of MDMA, MDEA, and MDA in whole blood, serum, vitreous humor, and urine. (C) 2000 American Association for Clinical Chemistry.

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Documento generato il 09/04/20 alle ore 00:16:29