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Titolo:
Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis
Autore:
Wang, J; Liu, XY; Jiang, W; Liang, L;
Indirizzi:
Beijing Univ, Sch Oncol, Dept Internal Med, Beijing 100036, Peoples R China Beijing Univ Beijing Peoples R China 100036 jing 100036, Peoples R China
Titolo Testata:
CHINESE MEDICAL JOURNAL
fascicolo: 11, volume: 113, anno: 2000,
pagine: 985 - 990
SICI:
0366-6999(200011)113:11<985:TAAEOT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Keywords:
telomerase activity; telomerase catalytic subunit gene; apoptosis; bcl-2; non-small cell lung cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, J Beijing Univ, Sch Oncol, Dept Internal Med, Beijing 100036, Peoples R China Beijing Univ Beijing Peoples R China 100036 036, Peoples R China
Citazione:
J. Wang et al., "Telomerase activity and expression of the telomerase catalytic subunit gene in non-small cell lung cancer: correlation with decreased apoptosis and clinical prognosis", CHIN MED J, 113(11), 2000, pp. 985-990

Abstract

Objective To investigate the level of telomerase activation (TA) and telomerase catalytic subunit (hEST(2)) gene mRNA in patients with non-small celllung cancer, and determine whether they are associated with tumor cell apoptosis, stage, clinical outcome. Methods Primary tumor specimens from 58 patients untreated with chemotherapy and 10 cases of histologically benign and adjacent lung tissue were analyzed. TA and hEST2 were measured by means of a modified telomerase repeat amplification protocol (TRAP) assay and in situ hybridization (ISH), respectively. Terminal deoxynucleotidyl transferase ( TdT)-mediated biotin dUTP nick end-labeling (TUNEL) was used to evaluate apoptotic cells. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect bcl-2 mRNA expression. Results TA and hEST2 were detected in 45 (77.6%) and 43 (74.1%) of 58 tumor specimens, respectively, and not detected in specimens of adjacent and benign lung tissue. One case expressed hEST(2), as a weak positive. Statistically significant positive association was found between the level of TA andhEST(2) (r=0.85, P=0.001). TA and hEST(2) were associated with tumor stage, but not associated with tumor grade, gender and patient age. Positive rate of bcl-2 mRNA was 38 (65.5%) of 58 tumor specimens. The mean apoptotic index in the bcl-2 positive group (9.5+/-1.3) was lower than that in the bcl-2 negative one (19.8+/-2.1, P<0.05), suggesting that apoptotic index may beinversely associated with bcl-2 expression (r= - 0.48, P = 0.041). Bcl-2 expression in the TA and hEST(2) positive group (92.1% and 89.4%) was higherthan that in the negative one (50.0% and 45.0%, P = 0.043 and P = 0.032, respectively). The apoptotic index was lower in the TA or hEST2 positive group (8.2 +/- 1.4, 10.7 +/- 1.1) then in the negative one (20.5 +/- 1.6, 24.2/-2.1, P<0.05). A statistically significant inverse association was found between TA or hEST(2) and apoptotic index (r = - 0.45, P = 0.02 and r = -0.51, P=0.001, respectively). Positive correlation was also detected between TA or hEST2 and bcl-2 expression (r = 0.86, P = 0.01 and r = 0.73, P = 0.024, respectively). The level of hEST(2) mRNA and apoptotic index were associated with clinical outcome in a multivariate cox regression analysis. Conclusions High TA and hEST(2) were frequently detected in primary non-small cell lung cancer untreated with chemotherapy, having high bcl-2 expression and a low tumor cell apoptotic rate. This suggests that both TA and hEST(2) are correlated with the deregulation of apoptosis. hEST(2) and apoptotic index have prognostic significance in patients with non-small cell lung cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:52:51